| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Staphylococcus | TCGTCCGCAGAGTTCAAGG | TGCGTCATCATGTTGCACC | 59.71 | 59.12 | 209 | 171 |
100.00%
|
14.29%
|
| Primer 2 | Staphylococcus | TCGTCCGCAGAGTTCAAGG | ATGCGTCATCATGTTGCACC | 59.71 | 59.55 | 210 | 171 |
100.00%
|
14.29%
|
| Primer 3 | Staphylococcus | CACGCACAGTTCATCCCAG | ATACGCTACCAACGCCAGC | 58.83 | 60.52 | 250 | 154 |
100.00%
|
14.29%
|
| Primer 4 | Staphylococcus | TGCCGATTGGTGCAGTAGG | CTAACGCAACTGCACCAGC | 60.08 | 59.79 | 282 | 154 |
100.00%
|
12.50%
|
| Primer 5 | Staphylococcus | TGCCGATTGGTGCAGTAGG | TAACGCAACTGCACCAGC | 60.08 | 58.66 | 281 | 154 |
100.00%
|
12.50%
|
| Primer 6 | Staphylococcus | TCGTCCGCAGAGTTCAAGG | GCGTCATCATGTTGCACCTC | 59.71 | 59.90 | 208 | 149 |
100.00%
|
14.29%
|
| Primer 7 | Staphylococcus | TGGCAGTTGGAACAGGTGC | CCACCAATTGGCGTAATGGC | 60.83 | 60.18 | 268 | 143 |
100.00%
|
12.50%
|
| Primer 8 | Staphylococcus | GCAGTTGGAACAGGTGCAT | CCACCAATTGGCGTAATGGC | 58.66 | 60.18 | 266 | 143 |
100.00%
|
12.50%
|
| Primer 9 | Staphylococcus | GATGGCAGTTGGAACAGGTG | CCACCAATTGGCGTAATGGC | 59.12 | 60.18 | 270 | 143 |
100.00%
|
12.50%
|
| Primer 10 | Staphylococcus | TGGAACAGGTGCATTTGGTG | CCACCAATTGGCGTAATGGC | 58.96 | 60.18 | 261 | 143 |
100.00%
|
14.29%
|