| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 71 | Streptococcus | TTTTGCTCGCCACGGTGA | GTGACGTTCGTTCAAGCGC | 60.20 | 60.44 | 253 | 103 |
100.00%
|
50.82%
|
| Primer 72 | Streptococcus | GCACGTGTTAAAGGTGGCG | ACAGCCAAGTCAGCAAGCA | 60.08 | 60.15 | 296 | 102 |
100.00%
|
40.98%
|
| Primer 73 | Streptococcus | TCGCAAAGAGACCATGGCA | AAACGATCCACGCGTCCA | 59.63 | 59.66 | 255 | 102 |
100.00%
|
34.43%
|
| Primer 74 | Streptococcus | ACAAACAAGGCATCATCCGTG | ACCAACATTCTTTTGGCGAGC | 59.46 | 60.00 | 209 | 102 |
100.00%
|
37.70%
|
| Primer 75 | Streptococcus | TTGGACGCGTCGTTTCTGA | AGCGGACGAGTTTCCATGA | 59.93 | 59.03 | 175 | 99 |
93.75%
|
24.59%
|
| Primer 76 | Streptococcus | CTCGTCGTCAAGCTCGTCA | ACGACAAGCGCTTCGTTG | 59.79 | 59.07 | 268 | 99 |
100.00%
|
40.98%
|
| Primer 77 | Streptococcus | ATCGGTGGCGAAGAAGACG | ACGATTGGGAAGCCAGGA | 60.15 | 58.18 | 200 | 99 |
94.74%
|
29.51%
|
| Primer 78 | Streptococcus | CGAAGTGCTTGAAGTACCTGC | TGGCGAGCTTCTTTGTCAGT | 59.54 | 59.89 | 294 | 99 |
100.00%
|
49.18%
|
| Primer 79 | Streptococcus | CGAAGTGCTTGAAGTACCTGC | GGCGAGCTTCTTTGTCAGT | 59.54 | 58.08 | 293 | 99 |
100.00%
|
49.18%
|
| Primer 80 | Streptococcus | TCAGCACACACTGACCGTC | ACAAGGGCACGGATTGAGT | 59.93 | 59.24 | 179 | 99 |
100.00%
|
57.38%
|