| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1721 | Thermobifida | ACAACCGCATCAGCCTCA | AGTTCGAGAGCCACCACAC | 59.25 | 59.63 | 250 | 65 |
100.00%
|
100.00%
|
| Primer 1722 | Thermobifida | ACAACCGCATCAGCCTCA | TACCCAGTGCGGTCTTGTG | 59.25 | 59.63 | 156 | 65 |
100.00%
|
100.00%
|
| Primer 1723 | Thermobifida | ACGCCTTCCAGACGTTCA | ATCATCAGCAGCAGCCAGG | 58.87 | 60.15 | 157 | 65 |
100.00%
|
100.00%
|
| Primer 1724 | Thermobifida | GAAACCGTCCAAGGGCTGA | GCGCATCGTAGAACCAGGA | 59.93 | 59.86 | 181 | 65 |
100.00%
|
100.00%
|
| Primer 1725 | Thermobifida | TACCTCGCCTACACCGTCA | CTTGCAGCACCATCAGCAG | 60.00 | 59.49 | 178 | 65 |
100.00%
|
100.00%
|
| Primer 1726 | Thermobifida | CCGCTTCGACACTGCTGTA | AGCACGTCAAAGTCGCTGA | 60.08 | 59.93 | 195 | 65 |
100.00%
|
100.00%
|
| Primer 1727 | Thermobifida | AACCCGTTCGACGACGAT | TCGGTCCAGTGGGTGTTGA | 59.04 | 60.76 | 164 | 65 |
100.00%
|
100.00%
|
| Primer 1728 | Thermobifida | CGTCGTCATCTTGGGGTGT | TTCTCGGTCGTAGCCCAGT | 59.71 | 60.30 | 163 | 65 |
100.00%
|
100.00%
|
| Primer 1729 | Thermobifida | TGCCCACGCTCTTCTTCA | TCCCGAACAGGCTGTTGAC | 58.85 | 59.93 | 180 | 65 |
100.00%
|
100.00%
|
| Primer 1730 | Thermobifida | TGCCCACGCTCTTCTTCA | GGCAATGGCAATCCCGAAC | 58.85 | 59.86 | 191 | 65 |
100.00%
|
100.00%
|