| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 61 | Thermobifida | TGCCGTTCATCGTGCTGA | AGTACGAGACGAGCGTGAC | 59.66 | 59.21 | 285 | 132 |
100.00%
|
100.00%
|
| Primer 62 | Thermobifida | TGCCGTTCATCGTGCTGA | TGACCACCGTCATCACCA | 59.66 | 58.12 | 270 | 132 |
100.00%
|
100.00%
|
| Primer 63 | Thermobifida | TGCCGTTCATCGTGCTGA | ACCACCGTCATCACCAGT | 59.66 | 58.11 | 268 | 132 |
100.00%
|
100.00%
|
| Primer 64 | Thermobifida | AGCTGCAACACGCCTTCA | AATCGTCAGGTCGCAGCA | 60.20 | 59.34 | 284 | 132 |
100.00%
|
100.00%
|
| Primer 65 | Thermobifida | AACCGGAAGCCCAAGTTCC | TACCAGCTGGCCTTGGTGA | 60.23 | 60.85 | 155 | 132 |
100.00%
|
100.00%
|
| Primer 66 | Thermobifida | AACCAGGCAGTCATCCAGG | CTAGGCCTGAACGCTGAGT | 59.31 | 59.11 | 246 | 132 |
100.00%
|
100.00%
|
| Primer 67 | Thermobifida | ACCGCAAAATCGTGCAGC | AGCCGTTCCTCTTCGAAGG | 59.74 | 59.41 | 205 | 132 |
100.00%
|
100.00%
|
| Primer 68 | Thermobifida | AACCCGTTCGACGACGAT | CGGTCCAGTGGGTGTTGAT | 59.04 | 59.63 | 163 | 130 |
100.00%
|
100.00%
|
| Primer 69 | Thermobifida | CCTCAACGAGGGCAAGGAT | ACTTGCGCAGTGCCTCAT | 59.40 | 59.97 | 269 | 130 |
100.00%
|
100.00%
|
| Primer 70 | Thermobifida | ACGGCTCCACCTACGAGAT | ACGATCGCCTGAGGAATGC | 60.08 | 60.23 | 226 | 130 |
100.00%
|
100.00%
|