| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2541 | Thermobispora | GGGACGAGTCAACGCTGAA | CGATGTACCCGTGCTCGTT | 60.01 | 60.15 | 285 | 45 |
100.00%
|
100.00%
|
| Primer 2542 | Thermobispora | AGCCGTCGTACACCGTCTA | TCGCAGGCTCTTGCAGTT | 60.38 | 59.57 | 284 | 45 |
100.00%
|
100.00%
|
| Primer 2543 | Thermobispora | GGCATGCCGAACCTGTACT | ATCGGGATGAGCACCTCCT | 60.08 | 60.07 | 215 | 45 |
100.00%
|
100.00%
|
| Primer 2544 | Thermobispora | ACCATCGCGAACTTCACCA | GGAGCAGGTCGATGGTCAT | 59.63 | 59.17 | 208 | 43 |
100.00%
|
100.00%
|
| Primer 2545 | Thermobispora | TTGCGATCTTCGGCTCGT | CCCTCGTTCCATCCTGCAT | 59.43 | 59.47 | 158 | 43 |
100.00%
|
100.00%
|
| Primer 2546 | Thermobispora | TTGCGATCTTCGGCTCGT | ACCCTCGTTCCATCCTGCA | 59.43 | 60.92 | 159 | 43 |
100.00%
|
100.00%
|
| Primer 2547 | Thermobispora | ACGGTGCGCAATTTCGTC | TGAAGAACTGCGAGCCGT | 59.45 | 59.27 | 286 | 43 |
100.00%
|
100.00%
|
| Primer 2548 | Thermobispora | CACGGTGCGCAATTTCGT | TGAAGAACTGCGAGCCGTT | 59.75 | 59.93 | 287 | 43 |
100.00%
|
100.00%
|
| Primer 2549 | Thermobispora | TACCGTGGAGTACAGCCCA | TGGTGGAGAGCTCGACGAT | 59.92 | 60.38 | 295 | 43 |
100.00%
|
100.00%
|
| Primer 2550 | Thermobispora | CAAGAAGCTGCGCACCA | AAGAGCACGTCCTCCTTGC | 58.22 | 60.00 | 159 | 43 |
100.00%
|
100.00%
|