| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Thermobispora | TTCCTCATGTCGTCGGTGC | AGGTTGAACCAGGCCTTCC | 60.08 | 59.54 | 212 | 106 |
100.00%
|
100.00%
|
| Primer 42 | Thermobispora | TGATCGCCAATGCCGTG | TTGTGGGAGTCGAGCTTGC | 58.06 | 60.30 | 247 | 106 |
100.00%
|
100.00%
|
| Primer 43 | Thermobispora | TCGGTGGCGTACGTCAAA | TCCAGCAGGTCGATGAGGA | 59.27 | 60.00 | 245 | 106 |
100.00%
|
100.00%
|
| Primer 44 | Thermobispora | ATCATCGAGCACGGCTTCC | AGCTTGCGGTAGTAGGTGC | 60.23 | 59.78 | 170 | 106 |
100.00%
|
100.00%
|
| Primer 45 | Thermobispora | AGAGGCCATCATCGAGCAC | AGCTTGCGGTAGTAGGTGC | 59.55 | 59.78 | 177 | 106 |
100.00%
|
100.00%
|
| Primer 46 | Thermobispora | ACGAGTGATCGCCATCCTC | AGGTCCATGGTCACCTTGC | 59.26 | 59.62 | 270 | 106 |
100.00%
|
100.00%
|
| Primer 47 | Thermobispora | AACGAGTGATCGCCATCCTC | AGGTCCATGGTCACCTTGC | 59.90 | 59.62 | 271 | 106 |
100.00%
|
100.00%
|
| Primer 48 | Thermobispora | ATCCTCAAGCGGAACCACC | AAGAGCAGGAAGTCGTGGC | 59.70 | 60.00 | 299 | 105 |
100.00%
|
100.00%
|
| Primer 49 | Thermobispora | TGCTGATCTACGAACGCGAG | AAGAGCAGGAAGTCGTGGC | 60.25 | 60.00 | 268 | 105 |
100.00%
|
100.00%
|
| Primer 50 | Thermobispora | TGAACGCGATCGAGAAGGC | TCTTGGCGACGTGCTTGT | 60.52 | 59.89 | 288 | 105 |
100.00%
|
100.00%
|