| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2021 | Thermosipho | GCGCAATAGCTATGGACCC | ACGGCTCGTGTCAAGAACA | 58.46 | 59.56 | 263 | 70 |
100.00%
|
50.00%
|
| Primer 2022 | Thermosipho | TGCACACAAGTAATGCCGAAA | AAAGCGCTGCGTTTGCC | 59.05 | 60.02 | 168 | 70 |
100.00%
|
50.00%
|
| Primer 2023 | Thermosipho | GGGAAGACGGGAGAAGTAGT | TTGACCGCAGCTTGGACA | 58.15 | 59.49 | 180 | 70 |
100.00%
|
50.00%
|
| Primer 2024 | Thermosipho | AGCAGGAGGAGCAGGAACA | TGCGCAAGTCCTTCAGGA | 60.54 | 58.85 | 246 | 70 |
100.00%
|
50.00%
|
| Primer 2025 | Thermosipho | TGCGGAAGGATAACACATGAG | TCCATGTGGATGCATACAACC | 58.09 | 58.35 | 204 | 70 |
100.00%
|
50.00%
|
| Primer 2026 | Thermosipho | GGCCATCGTCTTTGATTGAGC | ACTCCTGGTGCACCCAAA | 59.94 | 58.66 | 280 | 70 |
100.00%
|
50.00%
|
| Primer 2027 | Thermosipho | GGCCATCGTCTTTGATTGAGC | ACTCCTGGTGCACCCAAAA | 59.94 | 59.38 | 280 | 70 |
100.00%
|
50.00%
|
| Primer 2028 | Thermosipho | TTCAGGTCCAACCGCTGT | GACCTGTTCCTCCAACAAACA | 58.76 | 58.35 | 284 | 70 |
100.00%
|
50.00%
|
| Primer 2029 | Thermosipho | ACGCCAAACTCAACTGATGT | GCGCAGAAGTAAATGTGGTGT | 58.03 | 59.46 | 172 | 70 |
100.00%
|
50.00%
|
| Primer 2030 | Thermosipho | TCTGGAATCCACCGGTTGT | CCTCTTGCCATCCCACTAACA | 58.54 | 59.72 | 172 | 70 |
100.00%
|
50.00%
|