| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 21 | Thermosipho | GACACACGTGGAGGAACAATC | AGTTTGTTGTATGGTGCGGAT | 59.20 | 58.21 | 239 | 141 |
100.00%
|
50.00%
|
| Primer 22 | Thermosipho | ACACACGTGGAGGAACAATC | AGTTTGTTGTATGGTGCGGAT | 58.12 | 58.21 | 238 | 141 |
100.00%
|
50.00%
|
| Primer 23 | Thermosipho | TGACCCTGCTCCAGTAAGTG | CGCTGCCGTTGTATCAACAG | 59.02 | 59.90 | 265 | 140 |
100.00%
|
50.00%
|
| Primer 24 | Thermosipho | ATTGGTGGAGGCGATGGTG | TGACCACCTTCACCTGCAG | 60.08 | 59.55 | 254 | 140 |
100.00%
|
50.00%
|
| Primer 25 | Thermosipho | TGCACCAGAAGCAAGAGGC | AAAGCAAGTGCAGTTCCCC | 60.60 | 58.57 | 231 | 140 |
100.00%
|
50.00%
|
| Primer 26 | Thermosipho | GCCGAAGCACTCCAAAAGG | CAGCCTTTTCACCAGTTGCA | 59.42 | 59.25 | 251 | 138 |
100.00%
|
50.00%
|
| Primer 27 | Thermosipho | GCCGAAGCACTCCAAAAGG | CCGCACCAACATAATCAGCA | 59.42 | 58.91 | 290 | 138 |
100.00%
|
50.00%
|
| Primer 28 | Thermosipho | GCCGAAGCACTCCAAAAGG | TCCGCACCAACATAATCAGCA | 59.42 | 60.34 | 291 | 138 |
100.00%
|
50.00%
|
| Primer 29 | Thermosipho | AGTGTTGCAGGAACATCATGT | CCAAATCTTGCCGTTTGTGCA | 58.13 | 60.54 | 176 | 138 |
100.00%
|
50.00%
|
| Primer 30 | Thermosipho | AGTGTTGCAGGAACATCATGT | CAAATCTTGCCGTTTGTGCA | 58.13 | 58.15 | 175 | 138 |
100.00%
|
50.00%
|