| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Thermosipho | ACTTGCCCAACGTTTGCA | TCGTCAAAAGACAACCTGAGC | 58.39 | 58.79 | 192 | 105 |
100.00%
|
50.00%
|
| Primer 422 | Thermosipho | TACTTGCCCAACGTTTGCA | TCGTCAAAAGACAACCTGAGC | 58.20 | 58.79 | 193 | 105 |
100.00%
|
50.00%
|
| Primer 423 | Thermosipho | TTACTTGCCCAACGTTTGCA | TCGTCAAAAGACAACCTGAGC | 58.90 | 58.79 | 194 | 105 |
100.00%
|
50.00%
|
| Primer 424 | Thermosipho | TACTTGCCCAACGTTTGCAT | TCGTCAAAAGACAACCTGAGC | 58.68 | 58.79 | 193 | 105 |
100.00%
|
50.00%
|
| Primer 425 | Thermosipho | GGATCTTGGGCTATTTGGTGC | GCGTTTCTTTGAGCTCCCAT | 59.32 | 58.83 | 258 | 105 |
100.00%
|
50.00%
|
| Primer 426 | Thermosipho | ACGGTGTGTGTGAGGTTGT | AGGATGCGGAAGTGATGCA | 59.40 | 59.40 | 194 | 105 |
100.00%
|
50.00%
|
| Primer 427 | Thermosipho | AAGGTGCCGACCAATGTGG | GCCCTGTGCTGTCCTACAT | 60.60 | 59.40 | 151 | 105 |
100.00%
|
50.00%
|
| Primer 428 | Thermosipho | GCAGCAAGCATGGGAACT | TCGCCAAGCAATTCCACTG | 58.31 | 58.74 | 216 | 105 |
100.00%
|
50.00%
|
| Primer 429 | Thermosipho | TGCGGTAGTGGGTACAACT | AGGCGAGGCAGGCTTATTC | 58.25 | 59.85 | 172 | 105 |
100.00%
|
50.00%
|
| Primer 430 | Thermosipho | TACGGAAAAGGTGGAGCACT | CCAAACTCAACAGGAATCGCC | 58.95 | 59.80 | 161 | 105 |
100.00%
|
50.00%
|