| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1181 | Thermotoga | GCTGAACGATTTCGCCACG | GCGTTTTCCATCCAGCACC | 60.23 | 59.79 | 196 | 88 |
100.00%
|
60.00%
|
| Primer 1182 | Thermotoga | TGGGATCGGCACCAAACTC | ATCCACGCTGTCAAGGACG | 60.00 | 60.08 | 205 | 88 |
100.00%
|
80.00%
|
| Primer 1183 | Thermotoga | TCGCGAGGGCTTTGTTGA | GCCTGCGCTCTGTCTTTTG | 59.58 | 59.79 | 196 | 88 |
100.00%
|
80.00%
|
| Primer 1184 | Thermotoga | ACACTCCGCACGTTGACA | TGTGGATGTTCGGGTGACG | 59.50 | 60.01 | 265 | 88 |
100.00%
|
80.00%
|
| Primer 1185 | Thermotoga | AGAAGCGGTTGTCGAGGTG | CGCGTTTTCACTCTCCACG | 60.01 | 59.51 | 157 | 88 |
100.00%
|
80.00%
|
| Primer 1186 | Thermotoga | GGGAACAAGGAAGGGCACA | GGTCTGCAACCGTTGAACG | 59.85 | 59.72 | 210 | 88 |
100.00%
|
80.00%
|
| Primer 1187 | Thermotoga | GACGTGAGAGCAGAGGATGG | TTGCGAGCTTTCCGGTGA | 59.90 | 59.58 | 219 | 88 |
100.00%
|
60.00%
|
| Primer 1188 | Thermotoga | TTCTGGACGCAAGCACCA | GTTCCCTCTTCTGCGGTCA | 59.49 | 59.33 | 259 | 88 |
100.00%
|
40.00%
|
| Primer 1189 | Thermotoga | TGCGCTGGACTTTGTGGA | GTTCCCTCTTCTGCGGTCA | 59.49 | 59.33 | 294 | 88 |
100.00%
|
40.00%
|
| Primer 1190 | Thermotoga | CGGCAAAACGGTTGCACA | AACGCACTCTGCCCTTCA | 59.90 | 59.17 | 174 | 88 |
100.00%
|
100.00%
|