| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1321 | Thermotoga | AAAGTGGGCAACGGAGGA | ACTGTCAGCCTCGTCGTTG | 58.75 | 60.01 | 151 | 84 |
100.00%
|
100.00%
|
| Primer 1322 | Thermotoga | GGGGTGGTTCTTGCAAACG | ATTTCACGCACCGACGGT | 59.64 | 59.97 | 287 | 84 |
100.00%
|
100.00%
|
| Primer 1323 | Thermotoga | TGCCCTCATCTCCGTGGTA | CAACGACAACGCTGCGAA | 60.00 | 59.38 | 223 | 84 |
100.00%
|
100.00%
|
| Primer 1324 | Thermotoga | TGGTCACAGTCTCGGTGGA | GCTTCCACCGCTTCTCTGT | 60.15 | 60.00 | 225 | 84 |
100.00%
|
80.00%
|
| Primer 1325 | Thermotoga | ATTGTGCTGGCTTCCGGA | ATCCCCTCGACACCCAGAT | 59.24 | 59.69 | 209 | 84 |
100.00%
|
80.00%
|
| Primer 1326 | Thermotoga | ATTGTGCTGGCTTCCGGA | ACGACAATCCCCTCGACAC | 59.24 | 59.41 | 215 | 84 |
100.00%
|
80.00%
|
| Primer 1327 | Thermotoga | TCTTCAGGGGTGTCGAGCT | AACGGCTGCTGTGTCTGT | 60.23 | 59.49 | 266 | 84 |
100.00%
|
40.00%
|
| Primer 1328 | Thermotoga | AGCCCTCGAAGAAGCCAAC | ATCGGTTCTGGCCACGAAG | 60.00 | 60.08 | 193 | 84 |
100.00%
|
60.00%
|
| Primer 1329 | Thermotoga | TAAACGAAGCGCTCCGGA | TCGAGCTCGTTCATTTCCCC | 59.04 | 60.11 | 166 | 84 |
100.00%
|
80.00%
|
| Primer 1330 | Thermotoga | AACCTCACGTCGCCAATGT | TCCGGTTTTCGCACTGGT | 59.93 | 59.49 | 236 | 84 |
100.00%
|
80.00%
|