| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1531 | Thermotoga | CGTGAGATTGTCGCTGGAGA | TCGTCTGGCGACTTTGCA | 59.83 | 59.58 | 171 | 81 |
100.00%
|
80.00%
|
| Primer 1532 | Thermotoga | AGCCACGATCAGCCAACA | ACCCCATACCTCCTCGACA | 59.25 | 59.30 | 249 | 81 |
100.00%
|
40.00%
|
| Primer 1533 | Thermotoga | GCGGTCTCATCGACAGACA | TCGTTCCTGAAGCCGTGT | 59.49 | 58.87 | 154 | 81 |
100.00%
|
80.00%
|
| Primer 1534 | Thermotoga | ACACTCCGCACGTTGACA | GGATGTTCGGGTGACGCAT | 59.50 | 60.45 | 262 | 81 |
100.00%
|
80.00%
|
| Primer 1535 | Thermotoga | CCTGTGAATCAGCTCGCCA | CGACGATCTCCTTCGCCTT | 60.08 | 59.57 | 217 | 81 |
100.00%
|
80.00%
|
| Primer 1536 | Thermotoga | TCCTGTGAATCAGCTCGCC | CGACGATCTCCTTCGCCTT | 59.78 | 59.57 | 218 | 81 |
100.00%
|
80.00%
|
| Primer 1537 | Thermotoga | AGCCCTCTCCAGCCATTCT | CGACGATCTCCTTCGCCTT | 60.31 | 59.57 | 273 | 81 |
100.00%
|
80.00%
|
| Primer 1538 | Thermotoga | TTGAAAGGGCTCGAACGC | AGCAGAACCGTTGCCGAT | 58.35 | 59.65 | 254 | 81 |
100.00%
|
80.00%
|
| Primer 1539 | Thermotoga | ACACGTGTCTGTCACCGTG | AAGCCGAGCACGTGAACA | 60.23 | 59.89 | 193 | 81 |
100.00%
|
80.00%
|
| Primer 1540 | Thermotoga | TCCCGTGATGTTTGCCTGT | CCTTTCCAGCACTGCCAAC | 59.55 | 59.34 | 263 | 81 |
100.00%
|
100.00%
|