| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1651 | Thermotoga | ACGGCAAAACGGTTGCAC | TGCCCTTCATCGAGCGTT | 59.90 | 59.34 | 166 | 78 |
100.00%
|
100.00%
|
| Primer 1652 | Thermotoga | CGGCAAAACGGTTGCACA | GAAGAACGCACTCTGCCCT | 59.90 | 60.00 | 178 | 78 |
100.00%
|
100.00%
|
| Primer 1653 | Thermotoga | CAGTGCGGAAGGAAGGGTT | TGCTGCTTCTTTGCGTGC | 59.93 | 59.67 | 204 | 78 |
100.00%
|
60.00%
|
| Primer 1654 | Thermotoga | AGAAGCGGAAGAAGGTGCC | AACGGACTCGCGATCGTT | 60.00 | 59.44 | 151 | 78 |
100.00%
|
60.00%
|
| Primer 1655 | Thermotoga | AAGCTGAGAGCCACGGTTC | AACGGACTCGCGATCGTT | 60.00 | 59.44 | 291 | 78 |
100.00%
|
60.00%
|
| Primer 1656 | Thermotoga | GAAGCTGAGAGCCACGGTT | AACGGACTCGCGATCGTT | 60.00 | 59.44 | 292 | 78 |
100.00%
|
60.00%
|
| Primer 1657 | Thermotoga | GCCACGGTTCAGGAAGTGA | AACGGACTCGCGATCGTT | 59.93 | 59.44 | 282 | 78 |
100.00%
|
60.00%
|
| Primer 1658 | Thermotoga | TTCTTCGCGTGGCTCGTT | CCAAAAGCCAGCCATCACG | 59.97 | 59.79 | 186 | 78 |
100.00%
|
80.00%
|
| Primer 1659 | Thermotoga | TTCTTCGCGTGGCTCGTT | CGTTGGTGGGCTCTGTGAA | 59.97 | 60.23 | 247 | 78 |
100.00%
|
80.00%
|
| Primer 1660 | Thermotoga | GCCGTTTTGATGGCGTTCC | TGCCATCGTGTAGCATCCT | 60.44 | 58.79 | 162 | 78 |
100.00%
|
80.00%
|