| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1701 | Thermotoga | ACGGAGTGGCAGACATTGG | TCGGAAACTTCGTGGCGA | 60.00 | 59.27 | 168 | 76 |
100.00%
|
80.00%
|
| Primer 1702 | Thermotoga | AGGAATGTGTCACGGGTGG | GCGTAAGAGCCTTCAGCGA | 59.63 | 60.15 | 171 | 76 |
100.00%
|
80.00%
|
| Primer 1703 | Thermotoga | TCGGACCACGCAGCTTTT | TCGCGAGTCTTGCCATGT | 59.89 | 59.34 | 268 | 76 |
100.00%
|
80.00%
|
| Primer 1704 | Thermotoga | TCCGCTGGCAAGGCTTTT | TGCGCTTGCCATGAAAGG | 60.20 | 59.04 | 259 | 76 |
100.00%
|
60.00%
|
| Primer 1705 | Thermotoga | TCCGCTGGCAAGGCTTTT | GCGCTTGCCATGAAAGGA | 60.20 | 58.72 | 258 | 76 |
100.00%
|
60.00%
|
| Primer 1706 | Thermotoga | TCCGCTGGCAAGGCTTTT | TTGCGCTTGCCATGAAAGG | 60.20 | 59.71 | 260 | 76 |
100.00%
|
60.00%
|
| Primer 1707 | Thermotoga | CGATCATTGTGGGAGTGGC | GCTGGGCAAAGCAACATCC | 58.60 | 60.08 | 239 | 76 |
100.00%
|
60.00%
|
| Primer 1708 | Thermotoga | AGCTCACTCACGCGATGA | AAACTCACGGTGGCGTGA | 58.72 | 59.50 | 208 | 76 |
100.00%
|
40.00%
|
| Primer 1709 | Thermotoga | TCTCATCACACTCGCGTGG | GCGGACACGAGAAAGACGA | 59.79 | 60.08 | 177 | 76 |
100.00%
|
80.00%
|
| Primer 1710 | Thermotoga | AGCGCTCGATGCTTCCTT | CTGTGGGGTCGTGGTCTTT | 59.42 | 59.55 | 197 | 76 |
100.00%
|
80.00%
|