| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1731 | Thermotoga | TGGAGAGGGTGCTTCGTGA | TCTCTGCCGTCTTTCACCG | 60.53 | 59.71 | 226 | 76 |
100.00%
|
80.00%
|
| Primer 1732 | Thermotoga | ACCCTTCGCTCCTTTGTGG | AGCTCCCACTTGGCGTTT | 59.93 | 59.48 | 221 | 76 |
100.00%
|
60.00%
|
| Primer 1733 | Thermotoga | ATAGACGTTCAGGGTGCGC | AGCTCCCACTTGGCGTTT | 60.15 | 59.48 | 164 | 76 |
100.00%
|
60.00%
|
| Primer 1734 | Thermotoga | GGTGCGCTCTCGGTAAAGA | AGCTCCCACTTGGCGTTT | 59.79 | 59.48 | 152 | 76 |
100.00%
|
60.00%
|
| Primer 1735 | Thermotoga | TATGGGACCCTTCGCTCCT | AGCTCCCACTTGGCGTTT | 59.69 | 59.48 | 227 | 76 |
100.00%
|
60.00%
|
| Primer 1736 | Thermotoga | CCCTTCGCTCCTTTGTGGA | AGCTCCCACTTGGCGTTT | 59.63 | 59.48 | 220 | 76 |
100.00%
|
60.00%
|
| Primer 1737 | Thermotoga | GGACCCTTCGCTCCTTTGT | AGCTCCCACTTGGCGTTT | 59.62 | 59.48 | 223 | 76 |
100.00%
|
60.00%
|
| Primer 1738 | Thermotoga | TTCCCCTCGTCCTTTTCGC | TTCCGCTGGCCACGATTT | 60.00 | 59.97 | 174 | 76 |
100.00%
|
40.00%
|
| Primer 1739 | Thermotoga | GTCCGTTCGGAACACTGGA | TCCCCTTCTCTTGGCCTCA | 59.64 | 59.84 | 254 | 76 |
100.00%
|
40.00%
|
| Primer 1740 | Thermotoga | AGGTCCGTTCGGAACACTG | TCCCCTTCTCTTGGCCTCA | 59.63 | 59.84 | 256 | 76 |
100.00%
|
40.00%
|