| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1921 | Thermotoga | TTTGGGGTGGGAGACGGAT | CAACGACAACGCTGCGAA | 60.54 | 59.38 | 264 | 72 |
100.00%
|
100.00%
|
| Primer 1922 | Thermotoga | TGGTTCTTGTGCAAGGGGAA | CGTTGACGATGAAAGGAAGGA | 59.74 | 58.32 | 209 | 72 |
100.00%
|
40.00%
|
| Primer 1923 | Thermotoga | ACAGCGATCCCGAACTTGG | TTCGGCTCCAGCGTTGAA | 60.08 | 59.58 | 214 | 72 |
100.00%
|
80.00%
|
| Primer 1924 | Thermotoga | CAGCGATCCCGAACTTGGA | TTCGGCTCCAGCGTTGAA | 59.78 | 59.58 | 213 | 72 |
100.00%
|
80.00%
|
| Primer 1925 | Thermotoga | AGTGCTGGCTTTCGGTGAA | AAGAACCCGTCCGCAACA | 59.85 | 59.49 | 168 | 72 |
100.00%
|
80.00%
|
| Primer 1926 | Thermotoga | TCGCAACGTACGTTCTGCT | ACAAAGACACCGAGCGCA | 60.01 | 59.89 | 220 | 72 |
100.00%
|
80.00%
|
| Primer 1927 | Thermotoga | GGTGTTTGAGAGGCTCGCT | TGTCACCGCTCAGAACCAC | 60.00 | 59.93 | 209 | 72 |
100.00%
|
80.00%
|
| Primer 1928 | Thermotoga | TCGCACACGTTCCCATGT | AACCGCACGAAGTCTGCT | 59.58 | 59.58 | 183 | 72 |
100.00%
|
60.00%
|
| Primer 1929 | Thermotoga | TTGGAGTGACTGCTGCAGC | CCTGTTTGGGAACACCGCT | 60.60 | 60.53 | 243 | 72 |
100.00%
|
60.00%
|
| Primer 1930 | Thermotoga | TGTGGCGAAACCTGTGGT | AGCTCCGCATCCACCTTTC | 59.41 | 60.08 | 264 | 72 |
100.00%
|
40.00%
|