| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2041 | Thermotoga | AAGCTCCTCTCCAGGCAGA | TCACAACAACGGGAGGCT | 59.92 | 58.76 | 229 | 69 |
100.00%
|
40.00%
|
| Primer 2042 | Thermotoga | GCTGCTGTTCAGGCTCTCA | TCCCACCGCTTTTGCCTT | 60.00 | 59.80 | 177 | 69 |
100.00%
|
80.00%
|
| Primer 2043 | Thermotoga | AGGCTCTCAAAGGGGTGGA | TCCCACCGCTTTTGCCTT | 60.15 | 59.80 | 167 | 69 |
100.00%
|
80.00%
|
| Primer 2044 | Thermotoga | TCGACGAAGCAACGGACA | TGGCTTCCGCTTCACCTT | 59.28 | 59.16 | 225 | 69 |
100.00%
|
80.00%
|
| Primer 2045 | Thermotoga | TCGACGAAGCAACGGACAA | TTGCATGTTCGCTTCCGC | 59.93 | 59.44 | 254 | 69 |
100.00%
|
80.00%
|
| Primer 2046 | Thermotoga | TCTCGACGAAGCAACGGAC | TTGCATGTTCGCTTCCGC | 60.08 | 59.44 | 256 | 69 |
100.00%
|
80.00%
|
| Primer 2047 | Thermotoga | AGCAACGGACAAATGGGGT | TTGCATGTTCGCTTCCGC | 59.85 | 59.44 | 247 | 69 |
100.00%
|
80.00%
|
| Primer 2048 | Thermotoga | ATCCTGGGAGCGGACATCT | AGTCACGTGAGCTGGCTT | 60.07 | 58.86 | 162 | 69 |
100.00%
|
80.00%
|
| Primer 2049 | Thermotoga | AGATCCTGGGAGCGGACAT | AGTCACGTGAGCTGGCTT | 60.07 | 58.86 | 164 | 69 |
100.00%
|
80.00%
|
| Primer 2050 | Thermotoga | GACACTCGGGACCATGCTT | AACGTGCCCTTGGATCGAG | 59.70 | 60.08 | 161 | 69 |
100.00%
|
40.00%
|