| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 201 | Thermotoga | GTTCATGCGAACGGTCAGC | CCGCTTTTCGCATCGCAT | 59.87 | 59.60 | 271 | 127 |
100.00%
|
80.00%
|
| Primer 202 | Thermotoga | ATGCGAACGGTCAGCTCAT | CCGCTTTTCGCATCGCAT | 59.78 | 59.60 | 267 | 127 |
100.00%
|
80.00%
|
| Primer 203 | Thermotoga | ACAACGTGTCCGCACTGT | AGCCGTTCTGCTGCATCT | 59.81 | 59.33 | 151 | 127 |
100.00%
|
40.00%
|
| Primer 204 | Thermotoga | ACAACGTGTCCGCACTGT | AAGCCGTTCTGCTGCATCT | 59.81 | 60.00 | 152 | 127 |
100.00%
|
80.00%
|
| Primer 205 | Thermotoga | CTCGAGCGAGAACCCGATAG | AGGGGATTGGGGAGGAGAT | 59.76 | 58.95 | 260 | 127 |
100.00%
|
40.00%
|
| Primer 206 | Thermotoga | TCGAGCGAGAACCCGATAG | AGGGGATTGGGGAGGAGAT | 58.68 | 58.95 | 259 | 127 |
100.00%
|
40.00%
|
| Primer 207 | Thermotoga | TCGACAACGGTCAGCACA | ACCACTTCCGCTACGTTGG | 59.19 | 60.01 | 166 | 126 |
100.00%
|
80.00%
|
| Primer 208 | Thermotoga | CTTTCGCAGGGGAGAGCAT | ACTGTGCGACGGTGTTGT | 59.78 | 59.81 | 249 | 126 |
100.00%
|
80.00%
|
| Primer 209 | Thermotoga | CTTTCGCAGGGGAGAGCAT | ACTGTGCGACGGTGTTGT | 59.78 | 59.81 | 249 | 126 |
100.00%
|
80.00%
|
| Primer 210 | Thermotoga | TGGTGTTGCTCGGCATGT | TCATGGCGTTCGAGCTTCC | 59.89 | 60.45 | 249 | 126 |
100.00%
|
60.00%
|