| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2131 | Thermotoga | ACAACCTCAGCGATGCCA | TTCTTCGGGAACACCCAGC | 59.25 | 59.93 | 161 | 68 |
100.00%
|
80.00%
|
| Primer 2132 | Thermotoga | TGAACTGGCAAAACTGGCG | CAAGAGCAGCGATCTGTGC | 59.27 | 59.29 | 278 | 68 |
100.00%
|
40.00%
|
| Primer 2133 | Thermotoga | AGCTTCCGGACGGTGTTT | TCAGGTTCGGTGTTGCGT | 59.17 | 59.50 | 252 | 68 |
100.00%
|
80.00%
|
| Primer 2134 | Thermotoga | AAGCTTCCGGACGGTGTT | TCAGGTTCGGTGTTGCGT | 59.17 | 59.50 | 253 | 68 |
100.00%
|
80.00%
|
| Primer 2135 | Thermotoga | AAAGCTTCCGGACGGTGT | TCAGGTTCGGTGTTGCGT | 59.17 | 59.50 | 254 | 68 |
100.00%
|
80.00%
|
| Primer 2136 | Thermotoga | AACCGGAACCAACACCGT | ATTCTCCAGCAGAGCAGCG | 59.41 | 60.15 | 151 | 68 |
100.00%
|
80.00%
|
| Primer 2137 | Thermotoga | ACAGCGCTATTGCCAGGA | TCCAAACCAGCGTGTCCA | 59.01 | 59.09 | 277 | 68 |
100.00%
|
40.00%
|
| Primer 2138 | Thermotoga | AACCAATCCCGTGGCTCTG | TCCAAACCAGCGTGTCCA | 60.00 | 59.09 | 234 | 68 |
100.00%
|
40.00%
|
| Primer 2139 | Thermotoga | TGGAAGTTGGCATCGGCA | CGTGGAAACAACGCAGTCG | 59.57 | 60.08 | 203 | 68 |
100.00%
|
40.00%
|
| Primer 2140 | Thermotoga | AGCCTTCTCTCTCCCTGCT | AGCCCACAAAAGTGCCGT | 59.61 | 60.12 | 271 | 68 |
100.00%
|
100.00%
|