| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2251 | Thermotoga | AAGCGCTGAAAGAAGCCCT | TTCGACGGTGAGCCTCAAC | 59.93 | 60.01 | 152 | 66 |
100.00%
|
80.00%
|
| Primer 2252 | Thermotoga | AAGCGCTGAAAGAAGCCCT | CTTCGACGGTGAGCCTCAA | 59.93 | 59.71 | 153 | 66 |
100.00%
|
80.00%
|
| Primer 2253 | Thermotoga | TGCCCTCATCTCCGTGGTA | CAACGACAACGCTGCGAAA | 60.00 | 60.01 | 223 | 66 |
100.00%
|
100.00%
|
| Primer 2254 | Thermotoga | CACAGAAAGCACGGGACCT | ATCCACGCTGTCAAGGACG | 59.93 | 60.08 | 180 | 66 |
100.00%
|
80.00%
|
| Primer 2255 | Thermotoga | GAAAAGCCCACACCCGAGA | CCACCTGCACCTCAAACCT | 59.93 | 59.85 | 213 | 66 |
100.00%
|
80.00%
|
| Primer 2256 | Thermotoga | TGTTCCTCGGCACTCTCCT | TGTGAAACGAAAGGTCCCCA | 60.23 | 59.45 | 175 | 66 |
100.00%
|
80.00%
|
| Primer 2257 | Thermotoga | AAAGGATGGGCTCCCTGGA | TGTCGAACACCACCTTCGTT | 60.23 | 59.82 | 163 | 66 |
100.00%
|
80.00%
|
| Primer 2258 | Thermotoga | TCGAAAGGATGGGCTCCCT | TGTCGAACACCACCTTCGTT | 60.31 | 59.82 | 166 | 66 |
100.00%
|
80.00%
|
| Primer 2259 | Thermotoga | TCGAAAGGATGGGCTCCCT | GTGTCGAACACCACCTTCG | 60.31 | 58.78 | 167 | 66 |
100.00%
|
80.00%
|
| Primer 2260 | Thermotoga | AAACGGTTGCGCAGGTCA | TGCCCTTCATCGAGCGTT | 60.52 | 59.34 | 160 | 66 |
100.00%
|
100.00%
|