| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2501 | Thermotoga | TCCCGTACGGTGAGACGAA | AGCCTGCGCTGAAACCA | 60.30 | 59.51 | 159 | 61 |
100.00%
|
60.00%
|
| Primer 2502 | Thermotoga | TGGGTGGGTACATGGGGAA | ACGTGCAAGCCTCACCAA | 60.15 | 59.81 | 299 | 61 |
100.00%
|
40.00%
|
| Primer 2503 | Thermotoga | GTGCGGAAGGAAGGGTTGA | GCTGCTTCTTTGCGTGCT | 59.93 | 59.36 | 201 | 61 |
100.00%
|
60.00%
|
| Primer 2504 | Thermotoga | AGTGCGGAAGGAAGGGTTG | GCTGCTTCTTTGCGTGCT | 59.93 | 59.36 | 202 | 61 |
100.00%
|
60.00%
|
| Primer 2505 | Thermotoga | GGGGACCGAATTCTCGTGA | TTCCACCGTGCTTCTTGCT | 59.10 | 59.85 | 164 | 61 |
100.00%
|
80.00%
|
| Primer 2506 | Thermotoga | TGCAAGAAGACGCTCGGT | AAACCGGTGTGGGTGCAA | 59.27 | 60.04 | 181 | 61 |
100.00%
|
80.00%
|
| Primer 2507 | Thermotoga | CTGCAAGAAGACGCTCGGT | AAACCGGTGTGGGTGCAA | 60.37 | 60.04 | 182 | 61 |
100.00%
|
80.00%
|
| Primer 2508 | Thermotoga | AGGGGAAGCAACAAACGGT | TGGCACTGCAGACGATGA | 59.77 | 58.94 | 161 | 61 |
100.00%
|
80.00%
|
| Primer 2509 | Thermotoga | CGCGATGCACAGAGATCCA | TCAACCAGAACGAGCGCT | 60.23 | 59.27 | 210 | 61 |
100.00%
|
80.00%
|
| Primer 2510 | Thermotoga | TTGGCTACGAACTCTCCGG | GGATCGCAGCAAGGAAGGT | 59.11 | 60.08 | 291 | 61 |
100.00%
|
100.00%
|