| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2681 | Thermotoga | TCCAACTGCCGTCACACT | TTCGCGTTGTCCCATCCA | 58.77 | 59.26 | 183 | 57 |
100.00%
|
40.00%
|
| Primer 2682 | Thermotoga | TCGGGTATCCCTGTGTGGT | TCGTAGAACCGACGCTTCG | 59.92 | 59.87 | 235 | 57 |
100.00%
|
80.00%
|
| Primer 2683 | Thermotoga | CCGCTGATGTCGTAACGGA | ACGTGTCCAAGGTCTCCCA | 59.86 | 60.46 | 235 | 57 |
100.00%
|
80.00%
|
| Primer 2684 | Thermotoga | GGCCTCACCTTGCAGTACA | TCGAGTCCGTAACCGACCA | 59.63 | 60.30 | 161 | 57 |
100.00%
|
60.00%
|
| Primer 2685 | Thermotoga | GCAGTGGCCATGTTGGATG | ACGCAGCGATCAACACCA | 59.49 | 59.97 | 172 | 57 |
100.00%
|
60.00%
|
| Primer 2686 | Thermotoga | TGCGAGTGCCTTCAGCTT | ATTGCGATGGCGATTGCG | 59.57 | 59.67 | 273 | 57 |
100.00%
|
80.00%
|
| Primer 2687 | Thermotoga | AAGGGGAAGCACGTTCTCG | AGAGTCCAGCTTCTCCGGT | 60.00 | 59.92 | 204 | 57 |
100.00%
|
80.00%
|
| Primer 2688 | Thermotoga | TCCGAAATGAAGGAAGGCCA | GCAAACGAAGTGCCCATGT | 59.30 | 59.34 | 195 | 57 |
100.00%
|
40.00%
|
| Primer 2689 | Thermotoga | AACGGTCGGACGATCTTCG | TGGATGTGGTCGGGTCTCA | 59.86 | 60.23 | 158 | 57 |
100.00%
|
40.00%
|
| Primer 2690 | Thermotoga | ATGCACAGCTTGTTGCCG | TCTGGCGATCTCAGCTCCA | 59.35 | 60.38 | 158 | 57 |
100.00%
|
80.00%
|