| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2721 | Thermotoga | CGAGGCGAAGAATGAGGCT | TGGCGAGTCTCAGAACGT | 59.86 | 58.24 | 255 | 56 |
100.00%
|
80.00%
|
| Primer 2722 | Thermotoga | TTCCCCTCGTCCTTTTCGC | TTTCCGCTGGCCACGATT | 60.00 | 59.97 | 175 | 56 |
100.00%
|
40.00%
|
| Primer 2723 | Thermotoga | GCAGCGGTTCTGACAGGAA | ACCCTTGCTGCAAACCGT | 60.30 | 60.12 | 254 | 56 |
100.00%
|
60.00%
|
| Primer 2724 | Thermotoga | AAGCAGCGGTTCTGACAGG | ACCCTTGCTGCAAACCGT | 60.30 | 60.12 | 256 | 56 |
100.00%
|
60.00%
|
| Primer 2725 | Thermotoga | AAGGCACTCTTCGACGCTC | TTACCCTATGGCCTCCCGT | 60.08 | 59.68 | 291 | 56 |
100.00%
|
80.00%
|
| Primer 2726 | Thermotoga | AAAGGCACTCTTCGACGCT | TTACCCTATGGCCTCCCGT | 59.63 | 59.68 | 292 | 56 |
100.00%
|
80.00%
|
| Primer 2727 | Thermotoga | TTGGGGTGGGAGACGGATT | CAACGACAACGCTGCGAA | 60.54 | 59.38 | 263 | 56 |
100.00%
|
100.00%
|
| Primer 2728 | Thermotoga | AAGGTGCCCTCATCTCCGT | CAACGACAACGCTGCGAA | 60.61 | 59.38 | 227 | 56 |
100.00%
|
100.00%
|
| Primer 2729 | Thermotoga | CTCAGTTTTGCGCTGCAGG | GGTTCCATCTTCGAGCCGT | 60.08 | 59.78 | 225 | 56 |
100.00%
|
80.00%
|
| Primer 2730 | Thermotoga | ACGCCAGAGAGCACCTTTC | TTTCAGCGGTGCGTCGT | 60.00 | 59.93 | 194 | 56 |
100.00%
|
80.00%
|