| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 2801 | Thermotoga | TGGTGTTCCACGCACAGA | ACCCCACTTGTGAAGCGTT | 59.10 | 59.77 | 258 | 55 |
100.00%
|
80.00%
|
| Primer 2802 | Thermotoga | TGTCGTACTGCGCAACCT | AGAACTTTCGCAGCCGTGA | 59.27 | 59.93 | 163 | 55 |
100.00%
|
80.00%
|
| Primer 2803 | Thermotoga | CAGTGCGGAAGGAAGGGTT | GCTGCTTCTTTGCGTGCT | 59.93 | 59.36 | 203 | 55 |
100.00%
|
60.00%
|
| Primer 2804 | Thermotoga | TGGCTCACAAGTGCGACA | CGGTCCGCTTTCTCTGCTA | 59.50 | 59.49 | 232 | 55 |
100.00%
|
80.00%
|
| Primer 2805 | Thermotoga | TGTCGCCAGAACGAAGGA | GATGAGCCTTCCGTCGGTT | 58.55 | 59.78 | 154 | 55 |
100.00%
|
80.00%
|
| Primer 2806 | Thermotoga | AGGGCTCAGAATCGCGTT | AGCACGTTCGTGGTGTGT | 59.02 | 59.81 | 288 | 55 |
100.00%
|
40.00%
|
| Primer 2807 | Thermotoga | AGTTCTGGCCAACGGTGT | GAAGCAGCCTCGTAAGCCT | 59.08 | 59.78 | 192 | 55 |
100.00%
|
60.00%
|
| Primer 2808 | Thermotoga | ATGCTTCCCGTCCACGATG | AGTCACCGCACACGAGTT | 60.15 | 59.19 | 238 | 55 |
100.00%
|
80.00%
|
| Primer 2809 | Thermotoga | TCTGGACAACGTTTGCGGT | ACCCCTCATGGCGTATCCT | 60.15 | 60.07 | 223 | 55 |
100.00%
|
80.00%
|
| Primer 2810 | Thermotoga | GGACAACGTTTGCGGTCAG | ACCCCTCATGGCGTATCCT | 59.72 | 60.07 | 220 | 55 |
100.00%
|
80.00%
|