| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 301 | Thermotoga | TCGCGAATCTCTTCAGGGC | ACTGTGCGACGGTGTTGT | 59.86 | 59.81 | 171 | 121 |
100.00%
|
80.00%
|
| Primer 302 | Thermotoga | TCCGAAATGAAGGAAGGCCA | CCATGTGGCTCCGGATTGA | 59.30 | 59.78 | 182 | 121 |
100.00%
|
40.00%
|
| Primer 303 | Thermotoga | TGTTTTGCCCAGGTGCCA | ACCCAGGAGCTGCTTCTCA | 60.04 | 60.54 | 213 | 121 |
100.00%
|
40.00%
|
| Primer 304 | Thermotoga | ACCACCACAACCGCAACA | CGACCGTGTCTCCTCGTTT | 60.04 | 59.71 | 157 | 120 |
100.00%
|
80.00%
|
| Primer 305 | Thermotoga | TTGGAGTGACTGCTGCAGC | TGATTGCGTCGGGTGAACC | 60.60 | 60.67 | 219 | 120 |
100.00%
|
80.00%
|
| Primer 306 | Thermotoga | GACAGCGACAAAGAAGCGG | TGGCCATGTTGTGAGCCA | 59.51 | 59.48 | 292 | 119 |
100.00%
|
80.00%
|
| Primer 307 | Thermotoga | CGCTGCCATGGAAAAGGTG | AGTTCACGCCACCGACAA | 59.79 | 59.50 | 188 | 119 |
100.00%
|
80.00%
|
| Primer 308 | Thermotoga | AAACGTCGGTGCGGTGAT | AACACCAGCCACGACTGT | 59.97 | 59.09 | 280 | 119 |
100.00%
|
60.00%
|
| Primer 309 | Thermotoga | AAACGTCGGTGCGGTGAT | ACCCTTTCCAACCGTCACC | 59.97 | 59.85 | 214 | 119 |
100.00%
|
60.00%
|
| Primer 310 | Thermotoga | AAACGTCGGTGCGGTGAT | GAACACCAGCCACGACTGT | 59.97 | 60.23 | 281 | 119 |
100.00%
|
60.00%
|