| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3311 | Thermotoga | ATCGGACCACGCAGCTTT | TCGCGAGTCTTGCCATGT | 59.65 | 59.34 | 269 | 42 |
100.00%
|
80.00%
|
| Primer 3312 | Thermotoga | CGTGGTGATACTGATGCCGA | AACCACCACTGCTGCTGA | 59.90 | 59.08 | 194 | 42 |
100.00%
|
60.00%
|
| Primer 3313 | Thermotoga | GGTTCTCCCAGGTTGTCGA | GTTCCCGATGTCCAGCAGA | 58.94 | 59.40 | 155 | 42 |
100.00%
|
100.00%
|
| Primer 3314 | Thermotoga | TGTGCAGATTCGACGCGA | AGAGCGAGCTTGCGAACT | 59.74 | 59.35 | 235 | 42 |
100.00%
|
40.00%
|
| Primer 3315 | Thermotoga | TTGAGTTCGACGCCAGCA | TCACCGAACAAACCAGCGA | 59.58 | 59.86 | 181 | 42 |
100.00%
|
80.00%
|
| Primer 3316 | Thermotoga | CTTGACGGTGAAGAGCCCA | AAACTGCGATCCCAGCGA | 59.63 | 59.34 | 190 | 42 |
100.00%
|
80.00%
|
| Primer 3317 | Thermotoga | ACTTCAGAACAGCCGTCCC | CTCTGCCGTCTTTCACCGA | 59.63 | 59.71 | 258 | 42 |
100.00%
|
80.00%
|
| Primer 3318 | Thermotoga | AGGGCATGATCAGAGCGA | TCCGTCTTCGTCCTCCAGT | 58.04 | 59.93 | 173 | 42 |
100.00%
|
80.00%
|
| Primer 3319 | Thermotoga | AGGGCATGATCAGAGCGA | TCGAGTCCTCAGGCCAGAA | 58.04 | 59.92 | 228 | 42 |
100.00%
|
80.00%
|
| Primer 3320 | Thermotoga | ACTGGCAAAACTGGCGATG | GCACACTCAAGAGCAGCGA | 59.04 | 60.67 | 282 | 42 |
100.00%
|
40.00%
|