| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3521 | Thermotoga | GCTTGGGATGCACGTGTT | TTCCATCCCGATTGCCCAC | 58.65 | 60.08 | 183 | 33 |
100.00%
|
40.00%
|
| Primer 3522 | Thermotoga | AACCGTTCCAGCCGAGTT | TGTCGCTCGGTCTGTTGAG | 59.17 | 59.71 | 231 | 33 |
100.00%
|
60.00%
|
| Primer 3523 | Thermotoga | GGGAAGCTCTAACGCTGCA | AAGCCCTGTGCAGTTCCT | 60.08 | 58.74 | 299 | 33 |
100.00%
|
60.00%
|
| Primer 3524 | Thermotoga | CCGAAGCGAGGGGTTTCAT | ATGAGTGCCTCCGCTGTT | 60.08 | 58.93 | 229 | 33 |
100.00%
|
60.00%
|
| Primer 3525 | Thermotoga | ACGCGTCCGATCTTGGTT | CGAACACCTCGTCCAGCTT | 59.35 | 60.01 | 279 | 33 |
100.00%
|
40.00%
|
| Primer 3526 | Thermotoga | GGGAAAGAGACACCAGCCT | GGCTGGGCTCCTTTCAGAA | 58.93 | 59.62 | 165 | 33 |
100.00%
|
80.00%
|
| Primer 3527 | Thermotoga | AGGGACGGGACTTTCCACT | TGCCAGAGCCACGATGTT | 60.15 | 59.25 | 166 | 33 |
100.00%
|
80.00%
|
| Primer 3528 | Thermotoga | TGTTCGCTCAACTGCCCT | TCAGCCAGCTCCTTTGCA | 59.17 | 59.16 | 245 | 33 |
100.00%
|
80.00%
|
| Primer 3529 | Thermotoga | AACGTGGACGCAACGGTT | TCCACAAGACACACCGCA | 60.51 | 59.10 | 182 | 33 |
100.00%
|
40.00%
|
| Primer 3530 | Thermotoga | TTCTTCGCCACGCTTCCT | TTCCTGCACCCAGAACGAC | 59.26 | 59.93 | 269 | 33 |
100.00%
|
60.00%
|