| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3551 | Thermotoga | ACGCTCAGGCAAAGTGGA | ATCCGAAAACCCACGCGA | 59.17 | 59.66 | 241 | 31 |
100.00%
|
80.00%
|
| Primer 3552 | Thermotoga | CGCTCAGGCAAAGTGGAGA | ATCCGAAAACCCACGCGA | 60.00 | 59.66 | 240 | 31 |
100.00%
|
80.00%
|
| Primer 3553 | Thermotoga | GCGCTTTGTCGAAACCGA | ACACCGTCACCTTCACGAC | 59.07 | 59.93 | 227 | 31 |
100.00%
|
60.00%
|
| Primer 3554 | Thermotoga | TCGTGAAAACGGTGCTCGA | ACCACCCGTGCCAACAAA | 59.93 | 60.04 | 179 | 31 |
100.00%
|
80.00%
|
| Primer 3555 | Thermotoga | AGGAAGCGCACAGGGTTT | TCGAGCCACTTCCTCACCT | 59.48 | 60.23 | 241 | 31 |
100.00%
|
100.00%
|
| Primer 3556 | Thermotoga | ATCGGTGGATCGATCGCAC | TGGGAAGCGCAAGAACGA | 60.01 | 59.58 | 160 | 31 |
100.00%
|
100.00%
|
| Primer 3557 | Thermotoga | CATCGGTGGATCGATCGCA | TGGGAAGCGCAAGAACGA | 60.01 | 59.58 | 161 | 31 |
100.00%
|
100.00%
|
| Primer 3558 | Thermotoga | TGCATCGGTGGATCGATCG | TGGGAAGCGCAAGAACGA | 60.01 | 59.58 | 163 | 31 |
100.00%
|
100.00%
|
| Primer 3559 | Thermotoga | TGCCGTTCTGATACCAGCC | AGCCGTTTGCCATGACGA | 59.78 | 59.97 | 194 | 31 |
100.00%
|
80.00%
|
| Primer 3560 | Thermotoga | TTGCTCAGACCGCTGGAA | CCTGAACCGTGCCGAGTTT | 58.85 | 60.60 | 156 | 31 |
100.00%
|
80.00%
|