| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 3651 | Thermotoga | CCGGTTCCAAAGGGAGCAA | AACTCGGCTGGAACGGTT | 60.23 | 59.17 | 199 | 22 |
100.00%
|
60.00%
|
| Primer 3652 | Thermotoga | ACGAGGAGAGGCCATGAGT | GAAAGCCTCACGCCTTCCT | 60.00 | 60.00 | 167 | 22 |
100.00%
|
40.00%
|
| Primer 3653 | Thermotoga | AAGCCGTTCAGCGTGAGA | GCGAGGAATCGGAGCAGTT | 59.27 | 60.15 | 203 | 22 |
100.00%
|
60.00%
|
| Primer 3654 | Thermotoga | TTTCGTGGCTGTGAGACCG | AGCTCTTCGCGTGGTGTT | 60.30 | 59.58 | 243 | 22 |
100.00%
|
80.00%
|
| Primer 3655 | Thermotoga | CGCCTTTGAAGCTGCGTT | AGGTACCTGTCGAGCTGGA | 59.36 | 59.62 | 219 | 22 |
100.00%
|
40.00%
|
| Primer 3656 | Thermotoga | ACCCTCACATCTGGCTCGA | TGCTGTGTTCATGACCGCT | 60.31 | 59.93 | 276 | 20 |
100.00%
|
80.00%
|
| Primer 3657 | Thermotoga | ACCCTCACATCTGGCTCGA | GTGCTGTGTTCATGACCGC | 60.31 | 59.79 | 277 | 20 |
100.00%
|
80.00%
|
| Primer 3658 | Thermotoga | TTGCAAGGATGGGAGAGCC | TCACGAACCGCTTTCCGA | 59.70 | 59.27 | 288 | 20 |
100.00%
|
80.00%
|
| Primer 3659 | Thermotoga | GGTCCTGCCGTTAGCTTCA | TCGGGAAGAAGAGAGGCGA | 59.70 | 60.00 | 185 | 20 |
100.00%
|
80.00%
|
| Primer 3660 | Thermotoga | AGACGAAGCGATTGAGAAGGA | GCGCTGTCTTTCTCTTTTCGA | 59.18 | 59.21 | 155 | 20 |
100.00%
|
60.00%
|