| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 381 | Thermotoga | TTGCCCAGCTCAGCGAAT | CCACGTGCATCGGATGTCT | 59.65 | 60.15 | 297 | 116 |
100.00%
|
40.00%
|
| Primer 382 | Thermotoga | TTGCCCAGCTCAGCGAAT | GCAGCGATGATCTCCGTCT | 59.65 | 59.64 | 271 | 116 |
100.00%
|
40.00%
|
| Primer 383 | Thermotoga | AACTGGAGTGCGGAAGGTG | TGAGAAAGTGGCTCGCCTC | 59.93 | 59.71 | 259 | 116 |
100.00%
|
100.00%
|
| Primer 384 | Thermotoga | GGAACGGTTCTCTGCACCA | AGCCGTTCTGCTGCATCT | 59.93 | 59.33 | 226 | 116 |
100.00%
|
60.00%
|
| Primer 385 | Thermotoga | ACGACGTGTCCACACTGTG | AGCCGTTCTGCTGCATCT | 60.23 | 59.33 | 151 | 116 |
100.00%
|
60.00%
|
| Primer 386 | Thermotoga | AACGGTTCTCTGCACCACC | AGCCGTTCTGCTGCATCT | 60.23 | 59.33 | 224 | 116 |
100.00%
|
60.00%
|
| Primer 387 | Thermotoga | AGGGTGGCGAAGAAAGCA | CACGCCTTTTCTTGCAGCA | 59.16 | 59.64 | 159 | 116 |
100.00%
|
80.00%
|
| Primer 388 | Thermotoga | AGGGTGGCGAAGAAAGCA | GCCTTTTCTTGCAGCAGCC | 59.16 | 60.37 | 156 | 116 |
100.00%
|
60.00%
|
| Primer 389 | Thermotoga | AGGGTGGCGAAGAAAGCA | TCACGCCTTTTCTTGCAGC | 59.16 | 59.35 | 160 | 116 |
100.00%
|
100.00%
|
| Primer 390 | Thermotoga | AGGGTGGCGAAGAAAGCA | ACGCCTTTTCTTGCAGCAG | 59.16 | 59.34 | 158 | 116 |
100.00%
|
80.00%
|