| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 601 | Thermotoga | TCAAGATCGGCTTCTGGGC | GACAGGATCGCAGCAAGGA | 59.78 | 59.78 | 223 | 107 |
100.00%
|
100.00%
|
| Primer 602 | Thermotoga | TCAAGATCGGCTTCTGGGC | ACAGGATCGCAGCAAGGA | 59.78 | 58.61 | 222 | 107 |
100.00%
|
100.00%
|
| Primer 603 | Thermotoga | TCGTAACGCTGATCGGAGC | TTTCCGGTTGTACGCTCCC | 59.94 | 60.00 | 230 | 107 |
100.00%
|
80.00%
|
| Primer 604 | Thermotoga | AGAGGGAGATCGACAGGCT | TCCAGCGCTTCTCTCAACG | 59.38 | 60.08 | 251 | 106 |
100.00%
|
80.00%
|
| Primer 605 | Thermotoga | GTCACGCGAGATCCAGGAA | GGTGCTCTGTAACCTGACCC | 59.49 | 60.04 | 230 | 106 |
100.00%
|
40.00%
|
| Primer 606 | Thermotoga | GGATGCGGTGATAGCGGAA | TGCACGTCGCTTTCCACA | 59.93 | 60.20 | 216 | 106 |
100.00%
|
80.00%
|
| Primer 607 | Thermotoga | GGTCCAAACGGTAGCGGAA | TCAGCGGTTTCCTCGAGT | 60.00 | 58.23 | 275 | 106 |
100.00%
|
80.00%
|
| Primer 608 | Thermotoga | ACGTTGACACTGCAGGCT | TCGCGAGGTGGTAAGGAAC | 59.49 | 59.41 | 210 | 106 |
100.00%
|
80.00%
|
| Primer 609 | Thermotoga | TCATGCGAACGGTCAGCT | CCGCTTTTCGCATCGCAT | 59.34 | 59.60 | 269 | 106 |
100.00%
|
80.00%
|
| Primer 610 | Thermotoga | TTCATGCGAACGGTCAGCT | CCGCTTTTCGCATCGCAT | 60.01 | 59.60 | 270 | 106 |
100.00%
|
80.00%
|