| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 631 | Thermotoga | TCATGCGAACGGTCAGCT | CCGCTTTTCGCATCGCAT | 59.34 | 59.60 | 269 | 106 |
100.00%
|
80.00%
|
| Primer 632 | Thermotoga | TTCATGCGAACGGTCAGCT | CCGCTTTTCGCATCGCAT | 60.01 | 59.60 | 270 | 106 |
100.00%
|
80.00%
|
| Primer 633 | Thermotoga | ACACCGGTGTTCATGCGAA | CCGCTTTTCGCATCGCAT | 60.23 | 59.60 | 279 | 106 |
100.00%
|
80.00%
|
| Primer 634 | Thermotoga | AGAAGCGGTTGTCGAGGTG | TGCTGAAGTGCTCCCATGG | 60.01 | 60.00 | 204 | 106 |
100.00%
|
80.00%
|
| Primer 635 | Thermotoga | CAGGCAGTACAGGGTCGAA | TGGCTTTGGCGTGTTCCA | 59.03 | 60.12 | 185 | 106 |
100.00%
|
60.00%
|
| Primer 636 | Thermotoga | GGTCCAAACGGTAGCGGAA | TCAGCGGTTTCCTCGAGT | 60.00 | 58.23 | 275 | 106 |
100.00%
|
80.00%
|
| Primer 637 | Thermotoga | TACAGGTACGACCGGTGCT | TGGCGACGGGAATGTTGT | 60.30 | 59.57 | 221 | 106 |
100.00%
|
80.00%
|
| Primer 638 | Thermotoga | CGCTCTCCAAACACGAGCT | TGGCGACGGGAATGTTGT | 60.37 | 59.57 | 246 | 106 |
100.00%
|
80.00%
|
| Primer 639 | Thermotoga | AAACTCGGTGCGTCTCAGG | TCCGTGTTCACTGGCCTTC | 60.01 | 59.93 | 288 | 106 |
100.00%
|
80.00%
|
| Primer 640 | Thermotoga | CGATGGCATCAAAGCGGTG | TGCTTCCACCACGTGCAT | 59.94 | 59.89 | 259 | 105 |
100.00%
|
60.00%
|