| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 861 | Thermotoga | GGGTTTTGGAACGTGCTGG | TGTGAGGTACGCGTGTGAC | 59.64 | 60.01 | 298 | 97 |
100.00%
|
80.00%
|
| Primer 862 | Thermotoga | GAGCAACACAACGGGAAGC | AGCAGAACCGTTGCCGAT | 59.72 | 59.65 | 277 | 97 |
100.00%
|
80.00%
|
| Primer 863 | Thermotoga | AGCAACACAACGGGAAGC | AGCAGAACCGTTGCCGAT | 58.57 | 59.65 | 276 | 97 |
100.00%
|
80.00%
|
| Primer 864 | Thermotoga | AAGGGGAAGTTGTGGCGAG | CGAGTTCGCCTTCATCGGA | 59.93 | 59.86 | 180 | 96 |
100.00%
|
80.00%
|
| Primer 865 | Thermotoga | TGACACTCGTGTTCAGCCC | TGCTTCCACCACGTGCAT | 59.93 | 59.89 | 206 | 96 |
100.00%
|
60.00%
|
| Primer 866 | Thermotoga | ACGAACTCTCCGGATGGGA | AGCAGCAAGGAAGGTCACC | 60.00 | 59.93 | 281 | 96 |
100.00%
|
100.00%
|
| Primer 867 | Thermotoga | TTTTGTGGTGGGTGGGGA | TGTTTCCTGCGTGTGTGC | 58.56 | 58.89 | 154 | 96 |
100.00%
|
60.00%
|
| Primer 868 | Thermotoga | TGAACGATTTCGCCACGGA | GCGTTTTCCATCCAGCACC | 60.01 | 59.79 | 194 | 96 |
100.00%
|
80.00%
|
| Primer 869 | Thermotoga | TGCCCACTTACACGTTCAGG | TTCCACCACACGAGGAGGA | 60.25 | 60.15 | 177 | 96 |
100.00%
|
80.00%
|
| Primer 870 | Thermotoga | AGGAAATTAGCGCTGGAAGGA | TGAGGCATACCGTTTCCGAT | 59.44 | 59.17 | 152 | 96 |
100.00%
|
40.00%
|