| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Thermotoga | AGCACTTTCTTCTCCACCCG | ACGTGCTTCGCGAGAACA | 59.96 | 59.97 | 246 | 135 |
100.00%
|
80.00%
|
| Primer 82 | Thermotoga | AAAAGGGTGTCCGAGGTGC | TTCCATCCACCGCTACTGC | 60.23 | 59.78 | 152 | 135 |
100.00%
|
100.00%
|
| Primer 83 | Thermotoga | TTTCTCGCAGGTTGCCCA | CTTCGGCAAGTCGGAGTGT | 59.49 | 60.01 | 194 | 135 |
100.00%
|
40.00%
|
| Primer 84 | Thermotoga | TTTCTCGCAGGTTGCCCA | GGCAAGTCGGAGTGTTGGA | 59.49 | 59.93 | 190 | 135 |
100.00%
|
40.00%
|
| Primer 85 | Thermotoga | TTTCTCGCAGGTTGCCCA | TTCGGCAAGTCGGAGTGT | 59.49 | 58.87 | 193 | 135 |
100.00%
|
40.00%
|
| Primer 86 | Thermotoga | TTTCTCGCAGGTTGCCCA | GGAAAAGCTCCCACCACCA | 59.49 | 59.85 | 174 | 135 |
100.00%
|
40.00%
|
| Primer 87 | Thermotoga | TTTCTCGCAGGTTGCCCA | TGGAAAAGCTCCCACCACC | 59.49 | 59.85 | 175 | 135 |
100.00%
|
40.00%
|
| Primer 88 | Thermotoga | TTTCTCGCAGGTTGCCCA | TCTTCGGCAAGTCGGAGTG | 59.49 | 59.71 | 195 | 135 |
100.00%
|
40.00%
|
| Primer 89 | Thermotoga | TTTCTCGCAGGTTGCCCA | TCGGCAAGTCGGAGTGTTG | 59.49 | 60.30 | 192 | 135 |
100.00%
|
40.00%
|
| Primer 90 | Thermotoga | TTACGGCTTGCTCACGCA | AGAGAAGCTCCGAGTGCCA | 59.97 | 60.61 | 231 | 135 |
100.00%
|
40.00%
|