| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 251 | Thermus | ACGAGGAGAGGGAGCTGAT | GGAGGGGTTCCAAGACAAGC | 59.38 | 60.61 | 153 | 60 |
100.00%
|
50.00%
|
| Primer 252 | Thermus | TTGGTCCACGGCTTCCTCT | AGGTCGGGAAGGAGAAGGT | 60.84 | 59.53 | 191 | 60 |
100.00%
|
50.00%
|
| Primer 253 | Thermus | TTGGTCCACGGCTTCCTCT | TGCAGGTCGGGAAGGAGAA | 60.84 | 60.54 | 194 | 60 |
100.00%
|
50.00%
|
| Primer 254 | Thermus | TTGGTCCACGGCTTCCTCT | CTCCAGGTCCAAAAGCCAGG | 60.84 | 60.61 | 252 | 60 |
100.00%
|
50.00%
|
| Primer 255 | Thermus | TTGGTCCACGGCTTCCTCT | CCTCCAGGTCCAAAAGCCAG | 60.84 | 60.61 | 253 | 60 |
100.00%
|
50.00%
|
| Primer 256 | Thermus | TTGGTCCACGGCTTCCTCT | AGGTCGGGAAGGAGAAGGTA | 60.84 | 59.29 | 191 | 60 |
100.00%
|
50.00%
|
| Primer 257 | Thermus | TGGAGATCGTCTGTCCGGT | TGGTCCTCCTCGGAAACCT | 60.00 | 59.84 | 223 | 60 |
100.00%
|
50.00%
|
| Primer 258 | Thermus | AGAAGCGCATCAAGGCCT | TCGTCGGGAGAAAGGGGAA | 59.33 | 60.23 | 256 | 60 |
100.00%
|
50.00%
|
| Primer 259 | Thermus | GGCTTTGGGACCGCTACAT | TCCAGGTTCTCCATGCCGA | 60.08 | 60.61 | 253 | 59 |
100.00%
|
50.00%
|
| Primer 260 | Thermus | TGCGGATGGAAAGCGAGT | TGCCAAAGTCCACCACGA | 59.34 | 59.09 | 294 | 59 |
100.00%
|
50.00%
|