| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Thermus | TCGTGATGGTGGAGGGGAT | ATGGCCATGAGCTTGACCC | 60.00 | 60.08 | 235 | 50 |
100.00%
|
50.00%
|
| Primer 522 | Thermus | ATCCCGAGCTCCTCAAGGA | ACCTTGGTCACCGTTTCCC | 59.69 | 59.85 | 232 | 50 |
100.00%
|
50.00%
|
| Primer 523 | Thermus | ACGCCTGGATCTCCTCGTTC | TTGGCCAGGGAGATGAGGA | 61.67 | 59.92 | 193 | 50 |
100.00%
|
50.00%
|
| Primer 524 | Thermus | AGGAGGAGCCCAAGACCAA | ACTTGGCGAAGACCACGA | 60.15 | 58.87 | 162 | 49 |
100.00%
|
50.00%
|
| Primer 525 | Thermus | TTCTCCTCACCCACCACCA | GTGGTCCAGGAAAAGCCGA | 60.07 | 59.93 | 164 | 49 |
100.00%
|
50.00%
|
| Primer 526 | Thermus | AGGCCATTGACGAGCAGT | AGACGTCAATGCCCACGA | 58.93 | 58.94 | 192 | 49 |
100.00%
|
50.00%
|
| Primer 527 | Thermus | ACTTCCAGCACCTCCTCCA | AGAGGACGGTGTAGGGCAT | 60.15 | 60.00 | 156 | 49 |
100.00%
|
50.00%
|
| Primer 528 | Thermus | TTTCCCTCAAAGGCGACCG | TGACCTTGTTGCGCACCA | 60.30 | 60.12 | 156 | 49 |
100.00%
|
50.00%
|
| Primer 529 | Thermus | AGGCGTTGTACCTGGACCA | CGAGCTCCTTCTCCTCGTTG | 60.84 | 60.18 | 246 | 49 |
100.00%
|
50.00%
|
| Primer 530 | Thermus | TGGTCTACGACGTGGAGGT | TTGAAGCCGAACATGCGC | 59.93 | 59.44 | 226 | 49 |
100.00%
|
50.00%
|