| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Tsukamurella | TGTACAGCCTGCGCAAGT | TGGTGCTGTGAGTGCATGT | 59.57 | 59.85 | 190 | 143 |
100.00%
|
100.00%
|
| Primer 2 | Tsukamurella | TCGAATCCCGACGACGTTC | ATGCCCTTGCCACCGAT | 59.86 | 58.90 | 161 | 141 |
100.00%
|
100.00%
|
| Primer 3 | Tsukamurella | CGAGATCGATGCGCTGGAT | TTCCGGAGTTGTCGTGCTC | 60.08 | 60.01 | 269 | 141 |
100.00%
|
100.00%
|
| Primer 4 | Tsukamurella | CGACACCATCAAACGCGTG | TGCTCCTGCGCAAGAACT | 60.15 | 59.57 | 267 | 138 |
100.00%
|
100.00%
|
| Primer 5 | Tsukamurella | ATCAAACGCGTGGATGCC | TGCTCCTGCGCAAGAACT | 58.81 | 59.57 | 260 | 138 |
100.00%
|
100.00%
|
| Primer 6 | Tsukamurella | GACACCATCAAACGCGTGG | TGCTCCTGCGCAAGAACT | 59.79 | 59.57 | 266 | 138 |
100.00%
|
100.00%
|
| Primer 7 | Tsukamurella | CATCAAACGCGTGGATGCC | TGCTCCTGCGCAAGAACT | 60.23 | 59.57 | 261 | 138 |
100.00%
|
100.00%
|
| Primer 8 | Tsukamurella | CCATCAAACGCGTGGATGC | TGCTCCTGCGCAAGAACT | 60.23 | 59.57 | 262 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Tsukamurella | ACACCATCAAACGCGTGGA | TGCTCCTGCGCAAGAACT | 60.23 | 59.57 | 265 | 138 |
100.00%
|
100.00%
|
| Primer 10 | Tsukamurella | ACACCATCAAACGCGTGG | TGCTCCTGCGCAAGAACT | 58.66 | 59.57 | 265 | 138 |
100.00%
|
100.00%
|