| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Varibaculum | TGGTCTGGATGCATCAGGC | AAAGCCAGCACCGCTTCA | 59.77 | 60.20 | 262 | 121 |
100.00%
|
50.00%
|
| Primer 32 | Varibaculum | TGCGTACGCTGTCTGCAA | AACGCGAATCTGGCTGCA | 59.97 | 60.36 | 163 | 119 |
100.00%
|
50.00%
|
| Primer 33 | Varibaculum | TGCGTACGCTGTCTGCAA | AAACGCGAATCTGGCTGC | 59.97 | 59.13 | 164 | 119 |
100.00%
|
50.00%
|
| Primer 34 | Varibaculum | ACTCGCAAACCTGCCGAT | AGTGCGCTACCTGCTCTTG | 59.65 | 60.08 | 211 | 119 |
100.00%
|
50.00%
|
| Primer 35 | Varibaculum | TTGGAGTGGTGCAGCGAA | GCGACCTCCCCAAAATGGA | 59.49 | 60.00 | 239 | 117 |
100.00%
|
50.00%
|
| Primer 36 | Varibaculum | TTGGAGTGGTGCAGCGAA | TCGCGACCTCCCCAAAAT | 59.49 | 58.93 | 241 | 117 |
100.00%
|
50.00%
|
| Primer 37 | Varibaculum | TTGGAGTGGTGCAGCGAA | ATCGCGACCTCCCCAAAA | 59.49 | 58.93 | 242 | 117 |
100.00%
|
50.00%
|
| Primer 38 | Varibaculum | TTGGAGTGGTGCAGCGAA | AATCGCGACCTCCCCAAA | 59.49 | 58.93 | 243 | 117 |
100.00%
|
50.00%
|
| Primer 39 | Varibaculum | TTGGAGTGGTGCAGCGAA | AAATCGCGACCTCCCCAA | 59.49 | 58.93 | 244 | 117 |
100.00%
|
50.00%
|
| Primer 40 | Varibaculum | TGCGTACGCTGTCTGCAA | TCCAAACGCGAATCTGGCT | 59.97 | 60.00 | 167 | 117 |
100.00%
|
50.00%
|