| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Varibaculum | TGGCGACAATGTGGTGCT | GCCCAGATTTTCCCCACCA | 59.89 | 59.92 | 211 | 95 |
100.00%
|
50.00%
|
| Primer 82 | Varibaculum | CGTAGTGCTCGAAGCTGCT | TTCCAGATCAGCCTTGGCC | 60.15 | 59.70 | 172 | 95 |
100.00%
|
25.00%
|
| Primer 83 | Varibaculum | GGAAGTAGCCATGGACGCT | AGCCGATTCCGAATCCCAC | 59.48 | 59.85 | 179 | 95 |
100.00%
|
50.00%
|
| Primer 84 | Varibaculum | ATGCCCAAGATCTGCGCA | TCAATACCGCATCCGCTCC | 59.73 | 59.93 | 156 | 94 |
100.00%
|
50.00%
|
| Primer 85 | Varibaculum | ATGCCCAAGATCTGCGCA | ATACCGCATCCGCTCCTTG | 59.73 | 59.93 | 153 | 94 |
100.00%
|
50.00%
|
| Primer 86 | Varibaculum | TGACCGCTTCGAAAGAGCC | AGCTTTGCTCGGTGGCAT | 60.37 | 59.97 | 272 | 94 |
100.00%
|
50.00%
|
| Primer 87 | Varibaculum | AGAAACTGGCCAAGCGCA | ACCAACCCGTGTAGGCTTC | 60.20 | 59.63 | 272 | 94 |
100.00%
|
50.00%
|
| Primer 88 | Varibaculum | TGACCAGAAGCTGCTGCA | TGGTCTGCACGGCATAGAC | 59.17 | 59.78 | 203 | 94 |
100.00%
|
50.00%
|
| Primer 89 | Varibaculum | TGAAATGCTGGCCTCGCA | ATGGTGCCTTCTTCCAGCC | 59.97 | 60.00 | 162 | 94 |
100.00%
|
50.00%
|
| Primer 90 | Varibaculum | GGAAGTGGTGCGCAAACA | TCGGTTCACCAATGCGCA | 58.88 | 60.28 | 284 | 93 |
100.00%
|
25.00%
|