| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1251 | Virgibacillus | TTAGATGCGGACACTGGGG | ATGCACGCTGCTTTTGCC | 59.10 | 60.05 | 283 | 73 |
100.00%
|
100.00%
|
| Primer 1252 | Virgibacillus | ATTAGATGCGGACACTGGGG | ATGCACGCTGCTTTTGCC | 59.53 | 60.05 | 284 | 73 |
100.00%
|
100.00%
|
| Primer 1253 | Virgibacillus | GCGGAGGCACTAGAAATGC | CGCTCACGTGCTTCTTGT | 58.98 | 58.37 | 182 | 73 |
100.00%
|
100.00%
|
| Primer 1254 | Virgibacillus | TGCAGCAGATAACTCCGGA | CCCGTTGGTTGTCCGTTTC | 58.41 | 59.35 | 278 | 73 |
100.00%
|
100.00%
|
| Primer 1255 | Virgibacillus | CGAAGGTGGATGAGTGGGA | ACCACACCTGGGGAAATGG | 58.71 | 59.54 | 259 | 73 |
100.00%
|
100.00%
|
| Primer 1256 | Virgibacillus | ACGGATGGCTGGTTTGTGT | AAAAGCGCTGCATCCCAG | 59.85 | 58.72 | 236 | 73 |
100.00%
|
100.00%
|
| Primer 1257 | Virgibacillus | ACAGCGAAACGTTCCGGA | CGTTTGCGGTGTAGGGTTAAC | 59.58 | 59.80 | 168 | 73 |
100.00%
|
100.00%
|
| Primer 1258 | Virgibacillus | CCTTCTTGCAGTGGCTGGA | TTCGCCTTGGCAACCAGT | 59.93 | 59.81 | 292 | 73 |
100.00%
|
100.00%
|
| Primer 1259 | Virgibacillus | GGAAAACGAAAGCAGCGGA | CCATTCCCCTTCTCCTCCTC | 59.05 | 58.86 | 187 | 73 |
100.00%
|
100.00%
|
| Primer 1260 | Virgibacillus | TTCACCAAGCTGCTGGGAG | ACGGGACCTACGGTATGGA | 59.93 | 59.39 | 151 | 73 |
100.00%
|
100.00%
|