| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 41 | Wolinella | ACGCGATTCTTGCCTGCA | AGGATCGCATGTCCCAAGC | 60.36 | 60.15 | 299 | 82 |
100.00%
|
100.00%
|
| Primer 42 | Wolinella | ACGCGATTCTTGCCTGCA | TGCACTGCTCCATCACCTC | 60.36 | 59.70 | 247 | 82 |
100.00%
|
100.00%
|
| Primer 43 | Wolinella | TGGCAAGCCAACATCAGACT | GGCGTAGATCGAGCCTTGT | 59.89 | 59.56 | 156 | 82 |
100.00%
|
100.00%
|
| Primer 44 | Wolinella | GGCAAACATCACAGCGCA | GCGAGTAGGTGCATGGTGT | 59.36 | 60.08 | 179 | 82 |
100.00%
|
100.00%
|
| Primer 45 | Wolinella | AGCGGTCGTCAAAGGACTG | GCTGGAGCGGAGAATCATGA | 60.01 | 59.89 | 160 | 82 |
100.00%
|
100.00%
|
| Primer 46 | Wolinella | ACGCTTGCCTTCAGCCAT | TCGCCAAGCTCTCGTTCA | 59.97 | 58.95 | 218 | 82 |
100.00%
|
100.00%
|
| Primer 47 | Wolinella | ACGCTTGCCTTCAGCCAT | CTCGCCAAGCTCTCGTTCA | 59.97 | 60.08 | 219 | 82 |
100.00%
|
100.00%
|
| Primer 48 | Wolinella | TGTCGCTTTTGGTGGCCA | ACCAAGCGTGCCTCTTCA | 60.12 | 59.17 | 196 | 82 |
100.00%
|
100.00%
|
| Primer 49 | Wolinella | TGTCGCTTTTGGTGGCCA | AACCAAGCGTGCCTCTTCA | 60.12 | 59.85 | 197 | 82 |
100.00%
|
100.00%
|
| Primer 50 | Wolinella | TTCCGTGGACGAGATTGCC | GGAGTCTGCCCAAAGCCAT | 60.08 | 60.00 | 291 | 82 |
100.00%
|
100.00%
|