| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Yersinia | AGCCGTTCTCAGCGTGTT | ACGCAAACGCATCGCTTT | 59.58 | 59.36 | 264 | 70 |
100.00%
|
25.00%
|
| Primer 522 | Yersinia | ATCGCCAGCGTGCAAAAC | ACACAAGCCACGGTCTGT | 59.74 | 59.09 | 234 | 70 |
100.00%
|
35.71%
|
| Primer 523 | Yersinia | AGACCCACGTGTTGGCAT | AAGCGAAGGTCAGTTCCGG | 59.16 | 60.00 | 221 | 70 |
100.00%
|
25.00%
|
| Primer 524 | Yersinia | GAGCCAGCCACGGTAGTTT | TGAACAGGAACGCCACCA | 60.00 | 59.09 | 225 | 70 |
100.00%
|
42.86%
|
| Primer 525 | Yersinia | TGCTTCTTGCTCTTGCGGT | GCACGCTGAAACGCTTGT | 60.23 | 59.67 | 161 | 70 |
91.67%
|
39.29%
|
| Primer 526 | Yersinia | TGGCTGTTACTGCGTGCT | ACGGCAAGCTGTAACACCA | 59.57 | 59.85 | 245 | 70 |
100.00%
|
25.00%
|
| Primer 527 | Yersinia | TGCACAAAGCCCACTACGT | TGGCTTCTGACGCGAAGT | 59.85 | 59.27 | 198 | 70 |
100.00%
|
42.86%
|
| Primer 528 | Yersinia | GCAGCGAAAATCCGTCGTG | ACCGGTTGTCGCGTTGAA | 60.23 | 60.20 | 231 | 70 |
100.00%
|
28.57%
|
| Primer 529 | Yersinia | ATGCAGCTCACCCCAAGAG | CAGCTACCCCTTCCATCACA | 59.70 | 59.09 | 223 | 70 |
100.00%
|
28.57%
|
| Primer 530 | Yersinia | TGGCTGGAGGATGTCGAGA | ACGACCAGATTGCGCAGT | 60.00 | 59.66 | 197 | 69 |
100.00%
|
32.14%
|