| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 91 | Acidithiobacillus | ACAACCCGACTTTGCGCA | ATGTCGGGATTCTGCCAGC | 60.52 | 60.15 | 261 | 116 |
100.00%
|
50.00%
|
| Primer 92 | Acidithiobacillus | TTGCAGAAACAGCACGGC | TGTCCTGATTCGTGCGCA | 59.28 | 59.66 | 241 | 116 |
100.00%
|
50.00%
|
| Primer 93 | Acidithiobacillus | TGCAGACCATTCGCCACA | GGCATTTTCGGACCGAGGA | 59.57 | 60.08 | 205 | 115 |
100.00%
|
50.00%
|
| Primer 94 | Acidithiobacillus | TCGCGACGCCATGAAACA | TCCCATTCGTCGGGTTTGG | 60.36 | 60.00 | 225 | 115 |
100.00%
|
50.00%
|
| Primer 95 | Acidithiobacillus | TCGCGACGCCATGAAACA | TTCGTCGGGTTTGGTGAGG | 60.36 | 59.93 | 220 | 115 |
100.00%
|
50.00%
|
| Primer 96 | Acidithiobacillus | TCGCGACGCCATGAAACA | CGTCGGGTTTGGTGAGGAT | 60.36 | 59.70 | 218 | 115 |
100.00%
|
50.00%
|
| Primer 97 | Acidithiobacillus | TCTCCGGATGCGCAATGT | TGACCACCATACCGCACTG | 59.42 | 59.70 | 273 | 115 |
100.00%
|
50.00%
|
| Primer 98 | Acidithiobacillus | AACAAGCGCACCCTGGAA | ACAAAACCAGCACTGCGC | 59.81 | 59.59 | 221 | 114 |
100.00%
|
50.00%
|
| Primer 99 | Acidithiobacillus | TTTTGGTGGCATCGCGTG | CGGCATAATCATCGTGCGC | 59.36 | 60.15 | 230 | 114 |
100.00%
|
50.00%
|
| Primer 100 | Acidithiobacillus | TTGGGTTGAGTGGCTGCA | ACGGAGGTGCCCATACAGA | 59.40 | 60.31 | 278 | 114 |
100.00%
|
50.00%
|