| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 81 | Acidithiobacillus | AACAAGCGCACCCTGGAA | AAAACCAGCACTGCGCAAG | 59.81 | 59.93 | 219 | 117 |
100.00%
|
50.00%
|
| Primer 82 | Acidithiobacillus | TTTTGGTGGCATCGCGTG | ACCAGGTCAGTCCGATGGT | 59.36 | 60.23 | 189 | 117 |
100.00%
|
50.00%
|
| Primer 83 | Acidithiobacillus | TTTTGGTGGCATCGCGTG | GCGAGAACATCCACCAGGT | 59.36 | 59.70 | 201 | 117 |
100.00%
|
50.00%
|
| Primer 84 | Acidithiobacillus | TCCGACCGCAGACCAAAA | AGCGTCCCTTTGGGCAAT | 59.18 | 59.56 | 202 | 117 |
100.00%
|
50.00%
|
| Primer 85 | Acidithiobacillus | ATCCGACCGCAGACCAAA | AGCGTCCCTTTGGGCAAT | 58.93 | 59.56 | 203 | 117 |
100.00%
|
50.00%
|
| Primer 86 | Acidithiobacillus | GATCCGACCGCAGACCAAA | AGCGTCCCTTTGGGCAAT | 60.08 | 59.56 | 204 | 117 |
100.00%
|
50.00%
|
| Primer 87 | Acidithiobacillus | TCGCGACGATCTCCAAAGG | AGCGTCCCTTTGGGCAAT | 59.86 | 59.56 | 160 | 117 |
100.00%
|
50.00%
|
| Primer 88 | Acidithiobacillus | TTTCCTGCTGATCCGACCG | AGCGTCCCTTTGGGCAAT | 59.78 | 59.56 | 213 | 117 |
100.00%
|
50.00%
|
| Primer 89 | Acidithiobacillus | ATCCGACCGCAGACCAAAA | AGCGTCCCTTTGGGCAAT | 59.63 | 59.56 | 203 | 117 |
100.00%
|
50.00%
|
| Primer 90 | Acidithiobacillus | TTGGGTTGAGTGGCTGCA | GACCGATACGTGCCGAGAA | 59.40 | 59.57 | 261 | 117 |
100.00%
|
50.00%
|