| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 491 | Acidithiobacillus | TTCTGCGTTGCGGAGTGA | AGACGGCTTGCAGCACAT | 59.58 | 59.97 | 182 | 65 |
100.00%
|
50.00%
|
| Primer 492 | Acidithiobacillus | TGCTGCTCGCTTTCCTGA | CCGAGAATGACGAAGCCGA | 59.26 | 59.86 | 175 | 65 |
100.00%
|
50.00%
|
| Primer 493 | Acidithiobacillus | TTCGATGTGGTCACGCAGG | CGCGATGACCTGAAGCTGA | 60.37 | 60.15 | 162 | 65 |
100.00%
|
50.00%
|
| Primer 494 | Acidithiobacillus | GGGCATTACGAGGTACGCA | TGGCTGGACGCTGTGTTT | 59.86 | 59.81 | 181 | 64 |
100.00%
|
50.00%
|
| Primer 495 | Acidithiobacillus | AATCCACCAAGCAGCGCT | CGGTGATCGGCTGCACTAA | 59.97 | 60.15 | 264 | 63 |
100.00%
|
50.00%
|
| Primer 496 | Acidithiobacillus | GACATCCTGGTCGCCAACA | AGTGTTGCGCTGGCCATA | 60.00 | 59.65 | 210 | 63 |
100.00%
|
50.00%
|
| Primer 497 | Acidithiobacillus | GGCGACCAGATGCAGATGA | ACGTCGCAGTTTGCCCAA | 59.86 | 60.52 | 234 | 63 |
100.00%
|
50.00%
|
| Primer 498 | Acidithiobacillus | TGCGTGCGGTATGGACAT | TGGCGACAACCGATGGAT | 59.42 | 59.01 | 180 | 63 |
100.00%
|
50.00%
|
| Primer 499 | Acidithiobacillus | ATGCTTCGGTGACCTTCGG | ATCCGCAGCGTGCTGAAA | 60.08 | 60.36 | 245 | 63 |
100.00%
|
50.00%
|
| Primer 500 | Acidithiobacillus | TCCCGAAACCTTGCCCAA | TGCTGTTCCGCTTTAGCCA | 59.07 | 59.93 | 291 | 63 |
100.00%
|
50.00%
|