| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 501 | Acidithiobacillus | AGAACCGGTCAATGCGCA | ATGGGGTCGTGACGGGATA | 59.97 | 60.08 | 276 | 63 |
100.00%
|
50.00%
|
| Primer 502 | Acidithiobacillus | TAGCTCGGTTCGTCGCAA | AGCGCATGAACCAGCAGT | 59.04 | 59.97 | 177 | 63 |
100.00%
|
50.00%
|
| Primer 503 | Acidithiobacillus | CCGGACGAAACCCCTGAAA | TCCAGGTCAGTTGCGCAA | 59.93 | 59.49 | 175 | 63 |
100.00%
|
50.00%
|
| Primer 504 | Acidithiobacillus | TTTCCGGACGAAACCCCTG | TCCAGGTCAGTTGCGCAA | 59.93 | 59.49 | 178 | 63 |
100.00%
|
50.00%
|
| Primer 505 | Acidithiobacillus | ACTGCAGCGGTGGATGAAA | TCCAGGTCAGTTGCGCAA | 59.93 | 59.49 | 202 | 63 |
100.00%
|
50.00%
|
| Primer 506 | Acidithiobacillus | ATCCAGCGCTATGGCCAT | ACGTCCACAGGCCTTGAAG | 58.84 | 59.93 | 255 | 62 |
100.00%
|
50.00%
|
| Primer 507 | Acidithiobacillus | ACTTCTGCCCACGCTGAA | GCTTCCATCTCGCCAGTGA | 59.17 | 59.78 | 207 | 61 |
100.00%
|
50.00%
|
| Primer 508 | Acidithiobacillus | AAGCACCACTCGGCATGT | TTCACTGGCGATGCGGAA | 59.57 | 59.65 | 194 | 61 |
100.00%
|
50.00%
|
| Primer 509 | Acidithiobacillus | GTGCGTTTCGTGCTGGAA | AGCGGTGCAAACCAGTCA | 58.98 | 59.81 | 269 | 61 |
100.00%
|
50.00%
|
| Primer 510 | Acidithiobacillus | ACATGTTCCTGCACGGCT | TGCTGTGCTGGGCAAAGT | 59.57 | 60.12 | 234 | 61 |
100.00%
|
50.00%
|