| Primer | Genus | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Acidithiobacillus | CCATCGGCAGAACACTGGT | AGTGTTGACCCGTCTGCAG | 60.00 | 59.93 | 237 | 61 |
100.00%
|
50.00%
|
| Primer 522 | Acidithiobacillus | TCGACTTTCGACTGCGCA | GGCAGTGCATGAACAAGGC | 59.67 | 60.08 | 258 | 60 |
100.00%
|
50.00%
|
| Primer 523 | Acidithiobacillus | TCGACTTTCGACTGCGCA | GTGCATGAACAAGGCGTCC | 59.67 | 59.79 | 254 | 60 |
100.00%
|
50.00%
|
| Primer 524 | Acidithiobacillus | TCGTCCTGCGCAAGAACT | TGCTGACCACGTCATCCAC | 59.27 | 60.01 | 159 | 60 |
100.00%
|
50.00%
|
| Primer 525 | Acidithiobacillus | CGTGCGGAAATGCTCATGG | TTCATCTCGGCTTCGGCA | 59.94 | 59.02 | 281 | 60 |
100.00%
|
50.00%
|
| Primer 526 | Acidithiobacillus | ACGGAGCATGGCGAACAT | GCGAAAAGGTTGTCCGCA | 59.73 | 58.97 | 290 | 60 |
100.00%
|
50.00%
|
| Primer 527 | Acidithiobacillus | ATGCGTGCGGTATGGACA | TGGCGACAACCGATGGAT | 59.42 | 59.01 | 181 | 59 |
100.00%
|
50.00%
|
| Primer 528 | Acidithiobacillus | TCGACTTTCGACTGCGCA | GGGAGGCAGTGCATGAACA | 59.67 | 60.30 | 262 | 59 |
100.00%
|
50.00%
|
| Primer 529 | Acidithiobacillus | TGCACATTCAGGCTCAGGG | GGTTGACGAAGCCCTGGAA | 60.00 | 59.93 | 279 | 59 |
100.00%
|
50.00%
|
| Primer 530 | Acidithiobacillus | AGAACCGGTCAATGCGCA | TTGTGCGAGATCGATGGGG | 59.97 | 59.86 | 289 | 59 |
100.00%
|
50.00%
|