| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Aeromonas | TGCCAACAAAGCTGCTGC | AGTCACCGGTGTTACGCAG | 59.58 | 60.01 | 203 | 157 |
100.00%
|
50.00%
|
| Primer 2 | Aeromonas | TCTGTGGCGTGCTTCTCTG | GCAGCAACCAGAACAGCAC | 60.01 | 60.01 | 158 | 149 |
100.00%
|
62.50%
|
| Primer 3 | Aeromonas | TGGGGTTTGCCATGCTGT | GCCTTGAACGCATAGCTGC | 59.80 | 59.94 | 250 | 148 |
100.00%
|
75.00%
|
| Primer 4 | Aeromonas | TGGGGTTTGCCATGCTGT | CGCCTTGAACGCATAGCTG | 59.80 | 59.65 | 251 | 148 |
100.00%
|
50.00%
|
| Primer 5 | Aeromonas | AAGGGTTTGGCCATCGCA | GTTGCAGCGCTTCGAACT | 59.88 | 59.06 | 181 | 138 |
100.00%
|
50.00%
|
| Primer 6 | Aeromonas | AAGGGTTTGGCCATCGCA | GCAGCGCTTCGAACTTCTG | 59.88 | 59.87 | 178 | 138 |
100.00%
|
87.50%
|
| Primer 7 | Aeromonas | AAGGGTTTGGCCATCGCA | TGCAGCGCTTCGAACTTC | 59.88 | 58.76 | 179 | 138 |
100.00%
|
87.50%
|
| Primer 8 | Aeromonas | AAAAGCCTGTGCCCAGCA | AGGTGGAAGCCGAACTTGG | 60.12 | 59.93 | 249 | 138 |
100.00%
|
87.50%
|
| Primer 9 | Aeromonas | ATGATGAACGCGTCGGCA | TGTCCACGTTGAGGGTGTG | 60.13 | 59.85 | 169 | 138 |
100.00%
|
37.50%
|
| Primer 10 | Aeromonas | ATGATGAACGCGTCGGCA | GTGTCCACGTTGAGGGTGT | 60.13 | 59.85 | 170 | 138 |
100.00%
|
37.50%
|