| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 511 | Alkalihalobacillus | ACGTTCAGGTGCGCAAGT | GCATTAGCGCATCCACAGTT | 60.20 | 59.27 | 176 | 95 |
100.00%
|
50.00%
|
| Primer 512 | Alkalihalobacillus | TGATGCACGGCAGCTTGA | TCGTTTCAACCGCTCCCT | 59.97 | 58.86 | 194 | 95 |
100.00%
|
50.00%
|
| Primer 513 | Alkalihalobacillus | TGATGCACGGCAGCTTGA | ATCGTTTCAACCGCTCCCT | 59.97 | 59.32 | 195 | 95 |
100.00%
|
50.00%
|
| Primer 514 | Alkalihalobacillus | GTGCTAAAGCCCACGAGGA | TTATGCTGTCGCCTCCTGT | 59.70 | 58.71 | 224 | 95 |
100.00%
|
50.00%
|
| Primer 515 | Alkalihalobacillus | TCGGAAGGGCAATACAACCT | AAACGACTTGCCCTCCAAAA | 59.01 | 58.23 | 193 | 95 |
100.00%
|
50.00%
|
| Primer 516 | Alkalihalobacillus | TTCGGAAGGGCAATACAACCT | AAACGACTTGCCCTCCAAAA | 59.65 | 58.23 | 194 | 95 |
100.00%
|
50.00%
|
| Primer 517 | Alkalihalobacillus | AAGCTCGCTTATGCCCAGG | TCGGAATACCACCTGCTGC | 60.15 | 59.78 | 154 | 95 |
100.00%
|
50.00%
|
| Primer 518 | Alkalihalobacillus | AGAAGCAGGTCCAGGTGGA | TCGACATCTGCAACGCGAT | 60.15 | 60.15 | 261 | 95 |
100.00%
|
50.00%
|
| Primer 519 | Alkalihalobacillus | TGCTGAAGCGTTGGCACT | AGGGCTTGGACTCGTACCT | 60.20 | 59.92 | 213 | 95 |
100.00%
|
50.00%
|
| Primer 520 | Alkalihalobacillus | TGCCAAACGAGACGCGAT | TTGTTGTTGCCCACCGGA | 60.05 | 59.72 | 223 | 95 |
100.00%
|
50.00%
|