| Primer | Genus | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 521 | Alkalihalobacillus | AAGCCCAACGGACGATGA | ACGGTCATTTCGCAGCCT | 58.93 | 59.65 | 195 | 95 |
100.00%
|
50.00%
|
| Primer 522 | Alkalihalobacillus | GGCCTTGCATGCTAGGTCA | ACGGTCATTTCGCAGCCT | 60.08 | 59.65 | 159 | 95 |
100.00%
|
50.00%
|
| Primer 523 | Alkalihalobacillus | TCCCGACAACGAGCAATCC | TGGCTGCTTTACCTCCTGC | 60.08 | 60.00 | 294 | 95 |
100.00%
|
50.00%
|
| Primer 524 | Alkalihalobacillus | AGGGCAGTTACAAAATCCGGA | AGCTTGGACAATGCCTCAC | 59.65 | 58.05 | 189 | 95 |
100.00%
|
50.00%
|
| Primer 525 | Alkalihalobacillus | AGGGCAGTTACAAAATCCGGA | AAGCTTGGACAATGCCTCAC | 59.65 | 58.75 | 190 | 95 |
100.00%
|
50.00%
|
| Primer 526 | Alkalihalobacillus | TGCTTAGACGGAGGAGCCT | GCGGTTCGGTGCATTTGT | 60.00 | 59.36 | 287 | 95 |
100.00%
|
50.00%
|
| Primer 527 | Alkalihalobacillus | TGGCTGGAATGGCTGCAA | TGCCTCAATCGCGTCGTT | 59.88 | 60.05 | 158 | 95 |
100.00%
|
50.00%
|
| Primer 528 | Alkalihalobacillus | TGCGAGAGACATTGTCGAACA | TCGTCTAAATCCATCTCGCGT | 60.00 | 59.33 | 264 | 94 |
100.00%
|
50.00%
|
| Primer 529 | Alkalihalobacillus | ACGCCAGCTACGGACAAA | TTGCTGGCCACCACTCAA | 59.26 | 59.40 | 232 | 94 |
100.00%
|
50.00%
|
| Primer 530 | Alkalihalobacillus | TGCTTGGGGTTTGGCTGT | TCAGTCGTTGCACGCCAA | 59.72 | 60.20 | 213 | 94 |
100.00%
|
50.00%
|